Structure

Sulforhodamine B sodium salt, Inhibitor

Sulforhodamine B sodium salt

Purity

98.00%

Synonyms

Sulforhodamine B sodium salt; Acid Red 52; Kiton Red 620; inhibitor

Ordering
Cat. Num. AAA5755892 For Research Use Only!

Purity/Purification

98.00%

Form/Format

Solid. Powder

Solubility

DMSO: 50mg/mL (86.11mM) Need ultrasonic
H2O: 20mg/mL (34.44mM) Need ultrasonic
(<1mg/ml refers to the product slightly soluble or insoluble)

Formula

C27H29N2NaO7S2

SMILES

O=S(C1=CC=C(C2=C3C=CC(N(CC)CC)=CC3=[O+]C4=C2C=CC(N(CC)CC)=C4)C(S(=O)([O-])=O)=C1)([O-])=O.[Na+]

CAS Number

3520-42-1

Target & IC50

Others

In Vitro

Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via the determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell-based studies. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation, and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation.

Preparation and Storage

Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent

Structure

Related Product Information for Sulforhodamine B sodium salt, inhibitor

Sulforhodamine B sodium salt is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells.

References

Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.
Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

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Product Notes

The Sulforhodamine B sodium salt (Catalog #AAA5755892) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Sulforhodamine B sodium salt, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Sulforhodamine B sodium salt, Inhibitor