Rabbit anti-Human MYRIP Polyclonal Antibody | anti-MYRIP antibody

MYRIP, CT (MYRIP, SLAC2C, Rab effector MyRIP, Exophilin-8, Myosin-VIIa-and Rab-interacting protein, Synaptotagmin-like protein lacking C2 domains C) (Azide free) (HRP)

Cat. Num. AAA6323565

• Gene Names: MYRIP; SLAC2C; SLAC2-C
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYRIP; Polyclonal Antibody; CT (MYRIP; SLAC2C; Rab effector MyRIP; Exophilin-8; Myosin-VIIa-and Rab-interacting protein; Synaptotagmin-like protein lacking C2 domains C) (Azide free) (HRP); Synaptotagmin-like protein lacking C2 domains C; anti-MYRIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MYRIP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MYRIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 658-686 amino acids from the C-terminal region of human MYRIP.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MYRIP Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MYRIP antibody detected the MYRIP protein (arrow).)

Related Product Information for anti-MYRIP antibody

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments (By similarity).

Product Categories/Family for anti-MYRIP antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Nightingale, T.D., et al. Blood 113(20):5010-5018(2009). Hayasaka, S., et al. Asian J. Androl. 10(4):561-568(2008). Goehring, A.S., et al. J. Biol. Chem. 282(45):33155-33167(2007). Wu, C., et al. Proteomics 7(11):1775-1785(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 59853352
• NCBI GeneID: 25924
• NCBI Accession #: NP_056275.2
• NCBI GenBank Nucleotide #: NM_015460.3
• UniProt Accession #: Q8NFW9; Q32M41; Q32M42; Q569F7; Q8IUF5; Q9Y3V4; B3KWM3; B3KWW4; B7Z2H1; B7Z9V3; G3XAI8
• Molecular Weight: 83,948 Da
• NCBI Official Full Name: rab effector MyRIP isoform a
• NCBI Official Synonym Full Names: myosin VIIA and Rab interacting protein
• NCBI Official Symbol: MYRIP
• NCBI Official Synonym Symbols: SLAC2C; SLAC2-C
• NCBI Protein Information: rab effector MyRIP; exophilin-8; Slp homologue lacking C2 domains; slp homolog lacking C2 domains c; myosin-VIIa- and Rab-interacting protein; synaptotagmin-like protein lacking C2 domains C; synaptotagmin-like protein homologue lacking C2 domains-c
• UniProt Protein Name: Rab effector MyRIP
• Protein Family: Rab effector
• UniProt Gene Name: MYRIP
• UniProt Synonym Gene Names: SLAC2C; SlaC2-c; Slp homolog lacking C2 domains c
• UniProt Entry Name: MYRIP_HUMAN

Uniprot Description

MYRIP: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. Functions as a protein kinase A-anchoring protein (AKAP). May act as a scaffolding protein that links PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3p22.1

Cellular Component: transport vesicle; photoreceptor outer segment; perinuclear region of cytoplasm; exocyst; melanosome; synapse; actin cytoskeleton

Molecular Function: protein binding; zinc ion binding; protein kinase A binding; actin binding; Rab GTPase binding

Biological Process: intracellular protein transport; positive regulation of insulin secretion