Rabbit MYPT1 Polyclonal Antibody | anti-MYPT1 antibody

MYPT1, Phosphorylated (Thr853/Thr850) (Myosin Phosphatase 1) (HRP)

Cat. Num. AAA6489200

• Gene Names: PPP1R12A; MBSP; MYPT1
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYPT1; Polyclonal Antibody; Phosphorylated (Thr853/Thr850) (Myosin Phosphatase 1) (HRP); anti-MYPT1 antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes recombinant truncated MYPT1 phosphorylated on Thr850 (chicken, Q90623), MW ~80kD. Species Crossreactivity: human (Thr853, O014974), mouse (Thr852, Q9DBR7) and rat (Thr855, Q10728).
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MYPT1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot: Untreated C2C12 lysate (Lane 1), and pervanadate treated C2C12 lysate (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS616540 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).)

Western Blot (WB)

(Western Blot: ROKa/ROCK-II only (Lane 1), MYPT1 substrate (M9925-02) only (Lane 2), and MYPT1 substrate (M9925-02) phosphorylated by ROKa/ROCK-II (Lane 3) were probed with MBS616540 (0.5ug/ml))

Related Product Information for anti-MYPT1 antibody

Myosin phosphatase target subunit 1 (MYPT1), which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, as previously shown. To understand the role of MYPT1 in the regulation of the cytoskeleton in human diseases, a 4855-bp cDNA was cloned for human MYPT1 using the rat MYPT1 cDNA as probe. Sequencing analysis has revealed that human MYPT1 contains 1030 amino acid residues with a calculated molecular mass of approximately 115kD. Fluorescence in situ hybridization analysis placed the human MYPT1 gene on chromosome 12q15-q21.2. Radiation hybrid mapping has shown that the human MYPT1 gene is located very near the highly polymorphic marker CHLC.GATA65A12, which lies between D12S350 and D12S106.

Product Categories/Family for anti-MYPT1 antibody

Antibodies; Myosin

References

1. Wang, H., et al., J. Biol. Chem. 277: 30, 614-30, 621 (2002). 2. Genomics 44(1): 150-152 (1997). 3. Takahashi, N., et al., J. Cell Biol. 274: 37, 385-37, 390 (1999). 4. Totsukawa, G., et al., J. Cell. Biol. 144: 735-744 (1999). 5. Muranyi, A., et al., Biochem. J. 330(1): 225-231 (1998). 6. Kimura, K., et al., Science 273: 245-248 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 45384106
• NCBI GeneID: 396020
• NCBI Accession #: NP_990454.1
• NCBI GenBank Nucleotide #: NM_205123.1
• UniProt Accession #: Q90623; Q10727; Q90624
• Molecular Weight: 106,975 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Synonym Full Names: protein phosphatase 1 regulatory subunit 12A
• NCBI Official Symbol: PPP1R12A
• NCBI Official Synonym Symbols: MBSP; MYPT1
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 12A
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 12A
• UniProt Gene Name: PPP1R12A
• UniProt Synonym Gene Names: MBS; MYPT1; Myosin phosphatase target subunit 1

Uniprot Description

MYPT1: myosin phosphatase target subunit 1, a regulatory subunit of protein phosphatase 1. Also called the myosin-binding subunit of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. Four splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Cellular Component: A band; actin cytoskeleton; centrosome; cytosol; focal adhesion; kinetochore; PTW/PP1 phosphatase complex; Z disc

Molecular Function: 14-3-3 protein binding; enzyme inhibitor activity; phosphatase regulator activity; protein kinase binding

Biological Process: centrosome cycle; mitotic cell cycle; negative regulation of catalytic activity; positive regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; regulation of cell adhesion; regulation of nucleocytoplasmic transport; signal transduction