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Western Blot (WB) ((1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ENC1 Polyclonal Antibody | anti-ENC1 antibody

Goat anti-ENC1 Antibody

Gene Names
ENC1; NRPB; CCL28; ENC-1; PIG10; KLHL35; KLHL37; TP53I10
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ENC1; Polyclonal Antibody; Goat anti-ENC1 Antibody; ectodermal-neural cortex 1 (with BTB-like domain); CCL28; ENC-1; FLJ39259; KLHL35; KLHL37; NRPB; PIG10; TP53I10; OTTHUMP00000128235; OTTHUMP00000222054; ectoderm-neural cortex protein 1; ectodermal-neural cortex (with BTB-like domain); kelch-like 35; CCL28 antibody; ectodermal-neural cortex (with BTB-like domain) antibody; ectodermal-neural cortex 1 (with BTB-like domain) antibody; ectoderm-neural cortex protein 1 antibody; ENC1 antibody; ENC-1 antibody; FLJ39259 antibody; kelch-like 35 antibody; kelch-like 37 antibody; kelch-like protein 37 antibody; KLHL35 antibody; KLHL37 antibody; NRPB antibody; nuclear matrix protein NRP/B antibody; nuclear restricted protein; BTB domain-like (brain) antibody; OTTHUMP00000128235 antibody; OTTHUMP00000222054 antibody; p53-induced gene 10 protein antibody; PIG10 antibody; TP53I10 antibody; tumor protein p53 inducible protein 10 antibody; anti-ENC1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TSVSHDKLPKVQC
Sequence Length
589
Applicable Applications for anti-ENC1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence TSVSHDKLPKVQC, from the internal region of the protein sequence according to NP_003624.1; NP_001243505.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_003624.1; NP_001243505.1). Reported variants represent identical protein: NP_001243503.1, NP_001243504.1, NP_003624.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,854 Da
NCBI Official Full Name
ectoderm-neural cortex protein 1 isoform 1
NCBI Official Synonym Full Names
ectodermal-neural cortex 1
NCBI Official Symbol
ENC1
NCBI Official Synonym Symbols
NRPB; CCL28; ENC-1; PIG10; KLHL35; KLHL37; TP53I10
NCBI Protein Information
ectoderm-neural cortex protein 1
UniProt Protein Name
Ectoderm-neural cortex protein 1
UniProt Gene Name
ENC1
UniProt Synonym Gene Names
KLHL37; NRPB; PIG10; ENC-1
UniProt Entry Name
ENC1_HUMAN

NCBI Description

This gene encodes a member of the kelch-related family of actin-binding proteins. The encoded protein plays a role in the oxidative stress response as a regulator of the transcription factor Nrf2, and expression of this gene may play a role in malignant transformation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

ENC1: Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate- specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 5q13

Cellular Component: cell soma; cytoplasm; cytoskeleton; nuclear chromatin; nuclear matrix; nucleolus; nucleoplasm; nucleus

Molecular Function: actin binding; protein binding

Biological Process: multicellular organismal development; negative regulation of translation; nervous system development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination

Research Articles on ENC1

Similar Products

Product Notes

The ENC1 enc1 (Catalog #AAA422916) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ENC1 Antibody reacts with Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ENC1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ENC1 enc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TSVSHDKLPK VQC. It is sometimes possible for the material contained within the vial of "ENC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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