Rabbit anti-Human, Mouse Myosin Light Chain Kinase, Smooth Muscle Polyclonal Antibody | anti-MYLK antibody

Myosin Light Chain Kinase, Smooth Muscle, NT (smMLCK, MLCK, MLCK1, MLCK108, MLCK210, MYLK, MYLK1, DKFZp686I10125, FLJ12216, Kinase-related Protein, KRP, MSTP083, Telokin)

Cat. Num. AAA626497

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Myosin Light Chain Kinase; Smooth Muscle; Polyclonal Antibody; NT (smMLCK; MLCK; MLCK1; MLCK108; MLCK210; MYLK; MYLK1; DKFZp686I10125; FLJ12216; Kinase-related Protein; KRP; MSTP083; Telokin); Anti -Myosin Light Chain Kinase; anti-MYLK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MLCK. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MYLK antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human MLCK-long (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MLCK Antibody (M1) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the MLCK antibody detected the MLCK protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-MYLK antibody

MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Product Categories/Family for anti-MYLK antibody

Antibodies; Abs to Myosin

NCBI and Uniprot Product Information

• NCBI GI #: 45642714
• NCBI GeneID: 396445
• NCBI Accession #: NP_990790.1
• NCBI GenBank Nucleotide #: NM_205459.2
• UniProt Accession #: P11799; P19038; Q549S2; Q6LDG3
• Molecular Weight: 210,446 Da
• NCBI Official Full Name: myosin light chain kinase, smooth muscle
• NCBI Official Synonym Full Names: myosin, light chain kinase
• NCBI Official Symbol: MYLK
• NCBI Protein Information: myosin light chain kinase, smooth muscle; MLCK; telokin; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase (61-kDa active fragment)
• UniProt Protein Name: Myosin light chain kinase, smooth muscle
• Protein Family: Myosin light chain kinase
• UniProt Entry Name: MYLK_CHICK

Uniprot Description

smMLCK: Phosphorylates a specific serine in the N-terminus of a myosin light chain, which leads to the formation of calmodulin/MLCK signal transduction complexes which allow selective transduction of calcium signals

Protein type: EC 2.7.11.18; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; CAMK group; MLCK family

Cellular Component: membrane; cytosol

Molecular Function: calmodulin binding; metal ion binding; ATP binding; myosin light chain kinase activity

Biological Process: protein amino acid phosphorylation