Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Myosin Light Chain Kinase 3 (MYLK3) Polyclonal Antibody | anti-MYLK3 antibody
Polyclonal Antibody to Myosin Light Chain Kinase 3 (MYLK3)
Gene Names
Mylk3; MLCK; D830007F02Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Myosin Light Chain Kinase 3 (MYLK3); Polyclonal Antibody; Polyclonal Antibody to Myosin Light Chain Kinase 3 (MYLK3); anti-MYLK3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MYLK3. It has been selected for its ability to recognize MYLK3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-GYT VSQHEVLGGG RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP
MGHHHHHHSG SEF-GYT VSQHEVLGGG RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP
Sequence Length
819
Applicable Applications for anti-MYLK3 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MYLK3 (Gly488~Pro750) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071455
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,790 Da
NCBI Official Full Name
myosin light chain kinase 3 isoform 2
NCBI Official Synonym Full Names
myosin light chain kinase 3
NCBI Official Symbol
Mylk3
NCBI Official Synonym Symbols
MLCK; D830007F02Rik
NCBI Protein Information
myosin light chain kinase 3
UniProt Protein Name
Myosin light chain kinase 3
Protein Family
UniProt Gene Name
Mylk3
UniProt Synonym Gene Names
Cardiac-MLCK
Uniprot Description
caMLCK: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: CAMK group; EC 2.7.11.18; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 8|8 C3
Cellular Component: actin cytoskeleton; cytoplasm; cytosol
Molecular Function: ATP binding; calmodulin-dependent protein kinase activity; kinase activity; myosin light chain kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process: cardiac myofibril assembly; cellular response to interleukin-1; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; protein amino acid phosphorylation; regulation of vascular permeability during acute inflammatory response; sarcomere organization; sarcomerogenesis
Research Articles on MYLK3
Similar Products
Product Notes
The MYLK3 mylk3 (Catalog #AAA2004766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Myosin Light Chain Kinase 3 (MYLK3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin Light Chain Kinase 3 (MYLK3) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MYLK3 mylk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-GYT VSQHEVLGGG RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP. It is sometimes possible for the material contained within the vial of "Myosin Light Chain Kinase 3 (MYLK3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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