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Western Blot (WB) (Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse MYO1G Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Myosin IG Polyclonal Antibody | anti-MYO1G antibody

Polyclonal Antibody to Myosin IG (MYO1G)

Gene Names
Myo1g; E430002D17Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Myosin IG; Polyclonal Antibody; Polyclonal Antibody to Myosin IG (MYO1G); anti-MYO1G antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MYO1G. It has beenselected for its ability to recognize MYO1G in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRAHLIE LQRRFQAARQ PPLYGRDLVW PTPPAVLQPF QDTCRVLFSR WRARQLVKNI PPSDMIQIKA KVAAMGALQG LRQDWGCQRA WARDYLSSDT DNPTASHLFA EQLKALREKD GFGSVLFSSH VRKVNRFRKS RDRALLLTDR YLYKLEPGRQ YRVMRAVPLE AVTGLSVTSG RDQLVVLHAQ GYDDLVVCLH RSQPPLDNRI GELVGMLAAH CQGEGRTLEV RVSDCIPLSQ RGARRLISVE
Applicable Applications for anti-MYO1G antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
MYO1G (Val754~Thr1018)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant MYO1G (Val754~Thr1018) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2071527
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse MYO1G Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Spleen lysate; Primary Ab: 2ug/ml Rabbit Anti-Mouse MYO1G Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded spleen tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded spleen tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
117,227 Da
NCBI Official Full Name
unconventional myosin-Ig
NCBI Official Synonym Full Names
myosin IG
NCBI Official Symbol
Myo1g
NCBI Official Synonym Symbols
E430002D17Rik
NCBI Protein Information
unconventional myosin-Ig
UniProt Protein Name
Unconventional myosin-Ig
Protein Family
UniProt Gene Name
Myo1g

Uniprot Description

MYO1G: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Motor

Chromosomal Location of Human Ortholog: 11|11 A1

Cellular Component: cell projection; extracellular exosome; filopodium; lamellipodium; membrane; microvillus; myosin complex; phagocytic cup; plasma membrane

Molecular Function: actin binding; ATP binding; calmodulin binding; lipid binding; motor activity; nucleotide binding; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol-4,5-bisphosphate binding

Biological Process: adaptive immune response; cell gliding; cell-substrate adhesion; exocytosis; Fc-gamma receptor signaling pathway involved in phagocytosis; immune system process; phagocytosis; T cell mediated immunity; T cell migration

Research Articles on MYO1G

Similar Products

Product Notes

The MYO1G myo1g (Catalog #AAA2027842) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Myosin IG (MYO1G) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myosin IG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the MYO1G myo1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VRAHLIE LQRRFQAARQ PPLYGRDLVW PTPPAVLQPF QDTCRVLFSR WRARQLVKNI PPSDMIQIKA KVAAMGALQG LRQDWGCQRA WARDYLSSDT DNPTASHLFA EQLKALREKD GFGSVLFSSH VRKVNRFRKS RDRALLLTDR YLYKLEPGRQ YRVMRAVPLE AVTGLSVTSG RDQLVVLHAQ GYDDLVVCLH RSQPPLDNRI GELVGMLAAH CQGEGRTLEV RVSDCIPLSQ RGARRLISVE. It is sometimes possible for the material contained within the vial of "Myosin IG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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