Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) Antibody. The lane on the right is blocked with the phospho peptide.)
Rabbit anti-Human, Mouse Myogenic Factor 5 Polyclonal Antibody | anti-MYF5 antibody
Myogenic Factor 5 (Phospho-Ser49) Antibody
Gene Names
MYF5; bHLHc2
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
Myogenic Factor 5; Polyclonal Antibody; Myogenic Factor 5 (Phospho-Ser49) Antibody; MYF5; myogenic factor 5; anti-MYF5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
Myogenic Factor 5 (Phospho-Ser49) Antibody detects endogenous levels of Myogenic Factor 5 only when phosphorylated at Ser49.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
255
Applicable Applications for anti-MYF5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:1000
ELISA: 1:1000
Target Modification
Phospho
Modification Sites
H:S49; M:S49
Immunogen
The antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49.
Immunogen Range
21-70
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) Antibody. The lane on the right is blocked with the phospho peptide.)
Western Blot (WB)
(Western blot analysis of lysates from HeLa cells, using Myogenic Factor 5 (Phospho-Ser49) Antibody. The lane on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28 kDa
NCBI Official Full Name
myogenic factor 5
NCBI Official Synonym Full Names
myogenic factor 5
NCBI Official Symbol
MYF5
NCBI Official Synonym Symbols
bHLHc2
NCBI Protein Information
myogenic factor 5
UniProt Protein Name
Myogenic factor 5
Protein Family
UniProt Gene Name
MYF5
UniProt Synonym Gene Names
BHLHC2; Myf-5; bHLHc2
Uniprot Description
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Research Articles on MYF5
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Product Notes
The MYF5 myf5 (Catalog #AAA9502929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Myogenic Factor 5 (Phospho-Ser49) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Myogenic Factor 5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the MYF5 myf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myogenic Factor 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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