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Western Blot (WB) (MYO6 Antibody (C-term R1181) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MYO6 antibody detected the MYO6 protein (arrow).)

Rabbit anti-Human MYO6 Polyclonal Antibody | anti-MYO6 antibody

MYO6, CT (R1181) (MYO6, KIAA0389, Unconventional myosin-VI, Unconventional myosin-6) (AP)

Gene Names
MYO6; DFNA22; DFNB37
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MYO6; Polyclonal Antibody; CT (R1181) (MYO6; KIAA0389; Unconventional myosin-VI; Unconventional myosin-6) (AP); Unconventional myosin-6; anti-MYO6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-MYO6 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
MYO6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1173-1203 amino acids from the C-terminal region of human MYO6.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MYO6 Antibody (C-term R1181) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MYO6 antibody detected the MYO6 protein (arrow).)

Western Blot (WB) (MYO6 Antibody (C-term R1181) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the MYO6 antibody detected the MYO6 protein (arrow).)

Immunohistochemistry (IHC)

(MYO6 Antibody (C-term R1181) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYO6 Antibody (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (MYO6 Antibody (C-term R1181) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYO6 Antibody (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MYO6 Antibody (C-term R1181) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of MYO6 Antibody (C-term R1181) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-MYO6 antibody
This gene encodes a protein involved intracellular vesicle and organelle transport, especially in the hair cell of the inner ear. Mutations in this gene have been found in patients with non-syndromic autosomal dominant and recessive hearing loss.
Product Categories/Family for anti-MYO6 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Nishikawa, S., et al. Cell 142(6):879-888(2010). Cho, S.J., et al. J. Biol. Chem. 285(35):27159-27166(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Szczyrba, J., et al. Mol. Cancer Res. 8(4):529-538(2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
149,691 Da
NCBI Official Full Name
unconventional myosin-VI isoform 1
NCBI Official Synonym Full Names
myosin VI
NCBI Official Symbol
MYO6
NCBI Official Synonym Symbols
DFNA22; DFNB37
NCBI Protein Information
unconventional myosin-VI; unconventional myosin-6
UniProt Protein Name
Unconventional myosin-VI
Protein Family
UniProt Gene Name
MYO6
UniProt Synonym Gene Names
KIAA0389
UniProt Entry Name
MYO6_HUMAN

Uniprot Description

MYO6: a molecular motor involved in intracellular vesicle and organelle transport. It is one of the so-called unconventional myosins.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: Golgi apparatus; nuclear membrane; microvillus; lysosomal membrane; cytoplasmic membrane-bound vesicle; clathrin coated vesicle membrane; coated pit; DNA-directed RNA polymerase II, holoenzyme; cell cortex; clathrin-coated endocytic vesicle; cytosol; nucleoplasm; filamentous actin; ruffle; cell soma; membrane; perinuclear region of cytoplasm; endocytic vesicle; apical part of cell; axon; cytoplasm; unconventional myosin complex; plasma membrane; nucleus

Molecular Function: actin filament binding; calmodulin binding; minus-end directed microfilament motor activity; protein binding; motor activity; actin binding; ADP binding; ATP binding

Biological Process: response to drug; inner ear morphogenesis; DNA damage response, signal transduction by p53 class mediator; metabolic process; actin filament-based movement; regulation of synaptic plasticity; auditory receptor cell differentiation; regulation of secretion; locomotory behavior; endocytosis; synaptic transmission; intracellular protein transport; synaptogenesis; sensory perception of sound; glutamate secretion; dendrite development; positive regulation of transcription from RNA polymerase II promoter; protein targeting

Disease: Deafness, Autosomal Recessive 37; Deafness, Autosomal Dominant 22

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Product Notes

The MYO6 myo6 (Catalog #AAA6323462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYO6, CT (R1181) (MYO6, KIAA0389, Unconventional myosin-VI, Unconventional myosin-6) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYO6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MYO6 myo6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYO6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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