Rabbit MYH7 Polyclonal Antibody | anti-MYH7 antibody

Anti-MYH7 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109010

• Reactivity: Human, Cynomolgus
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MYH7; Polyclonal Antibody; Anti-MYH7 Antibody; Rabbit Polyclonal; MYH7 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CMD1S Antibody; Anti-CMH1 Antibody; Anti-MPD1 Antibody; Anti-MYHCB Antibody; Anti-SPMD Antibody; Anti-SPMM Antibody; anti-MYH7 antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MYH7
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MYH7 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 5-10uL/mg of lysate
• Immunogen: E Coli-derived Human MYH7 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MYH7 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MYH7 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:223 kDa Observed band size:250 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human MYH7 in human skeletal muscle with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MYH7 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(MYH7 was immunoprecipitated using: Lane A:0.5 mg Rat skeletal muscle Whole Cell Lysate 4 uL anti-MYH7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYH7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 223 kDa Observed band size :223 kDa)

Related Product Information for anti-MYH7 antibody

Mutations in the myosin heavy chain 7 (MYH7) gene are the source of an allelic series of diseases, including various cardiomyopathies and skeletal myopathies that usually manifest in adulthood. MYH7-related myopathies are emerging as an important group of muscle diseases of childhood and adulthood, with variable clinical and histopathological expression depending on the type and location of the mutation. Mutations in the head and neck domains are a well-established cause of hypertrophic cardiomyopathy whereas mutations in the distal regions have been associated with a range of skeletal myopathies with or without cardiac involvement, including Laing distal myopathy and Myosin storage myopathy. beta-myosin heavy chain (MHC) 7 (MYH7) is the dominant pathogenic gene that harbors mutations in 20-30% of cases of familial hypertrophic cardiomyopathy (HCM).

References

Wang B, et al. (2019) Genetic analysis of monoallelic double myh7 mutations responsible for familial hypertrophic cardiomyopathy. Mol Med Rep 20 (6): 5229-5238.Hershkovitz T, et al. (2019) Clinical diversity of myh7-related cardiomyopathies: Insights into genotype-phenotype correlations. Am J Med Genet A 179 (3): 365-372.Fiorillo C, et al. (2016) Myh7-related myopathies: Clinical, histopathological and imaging findings in a cohort of italian patients. Orphanet J Rare Dis 11 (1): 91.