Rabbit anti-Human, Mouse Myc Polyclonal Antibody | anti-Myc antibody

Myc Antibody

Cat. Num. AAA9407301

• Gene Names: POLE; FILS; POLE1; CRCS12
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Myc; Polyclonal Antibody; Myc Antibody; DNA polymerase epsilon catalytic subunit A; EC 2.7.7.7; DNA polymerase II subunit A; anti-Myc antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total POLE1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 2286

• Applicable Applications for anti-Myc antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human POLE1.
• Target Name: Myc
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using POLE1 antibody.)

Related Product Information for anti-Myc antibody

Participates in DNA repair and in chromosomal DNA replication.Kesti T., J. Biol. Chem. 268:10238-10245(1993).Syvaeoja J.E.;Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.Asahara H., Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.

Product Categories/Family for anti-Myc antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 62198237
• NCBI GeneID: 5426
• NCBI Accession #: NP_006222.2
• NCBI GenBank Nucleotide #: NM_006231.3
• UniProt Accession #: Q07864; Q13533; Q86VH9
• Molecular Weight: 261,518 Da
• NCBI Official Full Name: DNA polymerase epsilon catalytic subunit A
• NCBI Official Synonym Full Names: polymerase (DNA directed), epsilon, catalytic subunit
• NCBI Official Symbol: POLE
• NCBI Official Synonym Symbols: FILS; POLE1; CRCS12
• NCBI Protein Information: DNA polymerase epsilon catalytic subunit A
• UniProt Protein Name: DNA polymerase epsilon catalytic subunit A
• Protein Family: Mycosin
• UniProt Gene Name: POLE
• UniProt Synonym Gene Names: POLE1
• UniProt Entry Name: DPOE1_HUMAN

NCBI Description

This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]

Uniprot Description

POLE: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase type-B family.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: nucleoplasm; epsilon DNA polymerase complex; cytoplasm; plasma membrane; nucleus

Molecular Function: DNA binding; zinc ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin binding

Biological Process: DNA replication initiation; in utero embryonic development; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle

Disease: Colorectal Cancer, Susceptibility To, 12; Facial Dysmorphism, Immunodeficiency, Livedo, And Short Stature