Rabbit anti-Human mutY Polyclonal Antibody
mutY
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
mutY; Polyclonal Antibody; Anti -mutY; anti-mutY antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes MutY. The human peptide sequence has no significant homology with E. coli mutY (1).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Applicable Applications for anti-mutY antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:500-1:2000.
ELISA: 1:5000-20,000 using 50-100ng control peptide (M9559-01)/well.
Dilution: Western Blot: 1:500-1:2000.
ELISA: 1:5000-20,000 using 50-100ng control peptide (M9559-01)/well.
Immunogen
An 18-aa peptide near the N-terminus of human mutY (1). Peptide was coupled to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-mutY antibody
Escherichia coli MutY is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. The N-terminal domain of MutY, residues 1-226, has been shown to retain catalytic activity. The post-replicative adenine:8-oxo-7,8-dihydroguanine (GO) mismatch is crucial for G:C to T:A transversion. This mismatch is corrected by Escherichia coli MutY which excises the adenine from A:GO. A candidate gene coding for the human counterpart of MutY has been cloned as hMYH (535 aa) (1). Human MutY is approximately 55kD.
Product Categories/Family for anti-mutY antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The mutY (Catalog #AAA615290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The mutY reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's mutY can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:500-1:2000. ELISA: 1:5000-20,000 using 50-100ng control peptide (M9559-01)/well. Researchers should empirically determine the suitability of the mutY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mutY, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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