Western Blot (WB)
(All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: mouse testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit ENASE Polyclonal Antibody | anti-ENASE antibody
ENASE Antibody (Center)
Predicted: Dog
Predicted: Dog
IHC-P: 1:50-100
FC/FACS: 1:10-50
Western Blot (WB)
(All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: mouse testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilutionLane 1: HL-60 whole cell lysateLane 2: mouse testis lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)-Paraffin
(ENASE Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)-Paraffin
(ENASE Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(ENASE Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ENASE Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Background: Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytosolic enzyme which catalyzes the hydrolysis of peptides and proteins with mannose modifications to produce free oligosaccharides. [provided by RefSeq, Feb 2012]
Uniprot Description
Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
Research Articles on ENASE
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Product Notes
The ENASE engase (Catalog #AAA9232184) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENASE Antibody (Center) reacts with Human, Mouse Predicted: Dog and may cross-react with other species as described in the data sheet. AAA Biotech's ENASE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), ELISA (EIA). WB: 0.25 IHC-P: 1:50-100 FC/FACS: 1:10-50. Researchers should empirically determine the suitability of the ENASE engase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENASE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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