Rabbit anti-Human MuSK Polyclonal Antibody | anti-MUSK antibody

MuSK (Muscle Skeletal Receptor Tyrosine Kinase, MGC126323, MGC126324, Muscle Specific Kinase Receptor, Muscle Specific Tyrosine Protein Kinase Receptor, Receptor Tyrosine Kinase MuSK, Skeletal Muscle Receptor Tyrosine Kinase)

Cat. Num. AAA625476

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: MuSK; Polyclonal Antibody; MuSK (Muscle Skeletal Receptor Tyrosine Kinase; MGC126323; MGC126324; Muscle Specific Kinase Receptor; Muscle Specific Tyrosine Protein Kinase Receptor; Receptor Tyrosine Kinase MuSK; Skeletal Muscle Receptor Tyrosine Kinase); Anti -MuSK (Muscle Skeletal Receptor Tyrosine Kinase; anti-MUSK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MUSK.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-MUSK antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Human recombinant MUSK protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-MUSK Pab in mouse kidney tissue lysates (35ug/lane). MUSK(arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(MUSK Antibody flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MUSK Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-MUSK antibody

MuSK activates signaling cascades responsible for many aspects of synapse formation, including the organization of the postsynaptic membrane, synapse-specific transcription, and presynaptic differentiation. MuSK also mediates agrin-induced aggregation of acetylcholine receptors at the mature vertebrate neuromuscular junction and also during synapse formation. It has been shown that a majority of AChR Ab-seronegative myasthenia gravis patients possess serum autoantibodies against MuSK.

Product Categories/Family for anti-MUSK antibody

Antibodies; Abs to Receptors; Tyrosine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 261878457
• NCBI GeneID: 4593
• NCBI Accession #: NP_001159753.1
• NCBI GenBank Nucleotide #: NM_001166281.1
• UniProt Accession #: O15146; Q32MJ8; Q32MJ9; Q5VZW7; Q5VZW8
• Molecular Weight: 97,056 Da
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 3
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: MUSK
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase; muscle-specific kinase receptor; muscle-specific tyrosine-protein kinase receptor
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• Protein Family: Muskelin
• UniProt Gene Name: MUSK
• UniProt Synonym Gene Names: MuSK
• UniProt Entry Name: MUSK_HUMAN

NCBI Description

This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]

Uniprot Description

MUSK: a receptor tyrosine kinase that is essential for the establishment and maintenance of the neuromuscular junction (NMJ). Its activation by agrin, a neuronally derived heparan-sulfate proteoglycan, and the agrin receptor (LRP4), leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ. Its activation by agrin requires Dok7, which interacts with the cytoplasmic portion of MuSK and activates its tyrosine kinase activity.

Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; TK group; Musk family

Chromosomal Location of Human Ortholog: 9q31.3-q32

Cellular Component: postsynaptic membrane; integral to plasma membrane; cell junction; neuromuscular junction; receptor complex

Molecular Function: protein binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of neuron apoptosis; protein amino acid autophosphorylation; regulation of synaptic growth at neuromuscular junction; positive regulation of protein amino acid phosphorylation; cell differentiation; memory; transmembrane receptor protein tyrosine kinase signaling pathway; neuromuscular junction development

Disease: Myasthenic Syndrome, Congenital, 9, Associated With Acetylcholine Receptor Deficiency; Fetal Akinesia Deformation Sequence