Rabbit anti-Human, Mouse Muscle Glycogen Synthase Polyclonal Antibody | anti-GYS1 antibody

Anti-Human Muscle Glycogen Synthase Phospho-Ser640 Antibody

Cat. Num. AAA4157017

• Gene Names: GYS1; GSY; GYS
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity Purified
• Synonyms: Muscle Glycogen Synthase; Polyclonal Antibody; Anti-Human Muscle Glycogen Synthase Phospho-Ser640 Antibody; Human Muscle Glycogen Synthase; anti-GYS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This affinity purified antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1% by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 4

• Applicable Applications for anti-GYS1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: This phospho specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
• Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Testing Data

Related Product Information for anti-GYS1 antibody

Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3b that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 4262850
• NCBI GeneID: 2997
• NCBI Official Full Name: muscle glycogen synthase, partial
• NCBI Official Synonym Full Names: glycogen synthase 1
• NCBI Official Symbol: GYS1
• NCBI Official Synonym Symbols: GSY; GYS
• NCBI Protein Information: glycogen [starch] synthase, muscle
• Protein Family: Glycogen [starch] synthase

NCBI Description

The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]