CES2 / Carboxylesterase-2 Recombinant Protein | CES2 recombinant protein

Recombinant Mouse CES2 / Carboxylesterase-2 Protein (His tag)

Cat. Num. AAA2546940

• Gene Names: Ces2c; Ces2; CES 2; ACH M1; ces2A3
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: CES2 / Carboxylesterase-2; Recombinant Mouse CES2 / Carboxylesterase-2 Protein (His tag); ces2A3; CES2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 561

• Application Notes: The recombinant mouse CES2 consists of 542 amino acids and has a predicted molecular mass of 60.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCES2 is approximately 52 kDa.
• Predicted N Terminal: Gln 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CES2 recombinant protein

Background: Carboxylesterase 2 (CES2) is a member of the carboxylesterase family and belongs to the multigene family. Carboxylesterase 2 is responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and beroin. It also serves to hydrolyze long-chain fatty acid esters and thioesters. It is speculated that carboxylesterases may play a role in lipid metabolism and the blood-brain barrier system and together with isform 1, are a serine esterase involved in both drug metabolism and activation. Human carboxylesterase 2 is commonly expressed in tumor tissues and irinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors.

Description: A DNA sequence encoding the extracellular domain of mouse CES2 (NP_663578.1) (Met 1-Lys 557) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 21704206
• NCBI GeneID: 234671
• NCBI Accession #: NP_663578.1
• NCBI GenBank Nucleotide #: NM_145603.2
• UniProt Accession #: Q91WG0
• Molecular Weight: 62,470 Da
• NCBI Official Full Name: acylcarnitine hydrolase
• NCBI Official Synonym Full Names: carboxylesterase 2C
• NCBI Official Symbol: Ces2c
• NCBI Official Synonym Symbols: Ces2; CES 2; ACH M1; ces2A3
• NCBI Protein Information: acylcarnitine hydrolase
• UniProt Protein Name: Acylcarnitine hydrolase
• Protein Family: Liver carboxylesterase
• UniProt Gene Name: Ces2c
• UniProt Synonym Gene Names: Ces2; ACH M1; CES 2

NCBI Description

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. The transcription of this gene is regulated by several factors including HNF-4alpha (hepatocyte nuclear factor-4alpha), Sp1 (specificity protein 1), Sp3 and USF1 (upstream stimulatory factor 1). The expression and activity of this gene is age-related but independent of growth hormone level. This gene is clustered with several family members including a few of pseudogenes and Ces5 on chromosome 8.[provided by RefSeq, Jun 2010]

Uniprot Description

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; EC 3.1.1.56; EC 3.1.1.84; Endoplasmic reticulum; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 8|8 D3

Cellular Component: extracellular space; intracellular membrane-bound organelle

Molecular Function: acylcarnitine hydrolase activity

Biological Process: response to acid