Rabbit MUNC-18 Polyclonal Antibody | anti-MUNC-18 antibody
RABBIT ANTI MUNC-18 (pSer515)
Gene Names
Stxbp1; Sec1; nSec1; NSEC1A; n-sec1; rbSec1; ANC18HA; Unc18-1; rbSec1A; rbSec1B; Munc18-1
Reactivity
Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence)
Applications
Western Blot
Synonyms
MUNC-18; Polyclonal Antibody; RABBIT ANTI MUNC-18 (pSer515); anti-MUNC-18 antibody
Host
Rabbit
Reactivity
Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence)
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises Munc-18 phosphorylated at serine 515, a 67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C.
Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release.
Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Sequence Length
594
Applicable Applications for anti-MUNC-18 antibody
Western Blot (WB)*
Application Notes
Western Blot: This item detects a band/s of approximately 65kDa in rat cortex cell lysates
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
50% Glycerol
0.01% Bovine Serum Albumin
50% Glycerol
0.01% Bovine Serum Albumin
Immunogen
Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated Ser515 of Munc-18.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to rat Munc-18 (pSer515) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Shelf Life: 12 months from date of despatch.
Related Product Information for anti-MUNC-18 antibody
MBS221521 recognises Munc-18 phosphorylated at serine 515, a 67 kDa member of the STXBP/UNC-18/SEC1 family. It is neural specific, expressed in the brain, spinal cord and at very high levels in axons. Munc-18 is crucial to calcium-dependent neurotransmitter release, facilitating synaptic vesicle docking and fusion. It inhibits the formation of soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex by binding syntaxin 1 preventing the other components (synaptobrevin and synaptosomal associated protein of 25 kDa (SNAP-25)) from binding. The function of Munc-18 is regulated by the phosphorylation of serine 515 by protein kinase C. Munc-18 is also involved in the onset of female puberty through its regulation of glutamate release.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,736 Da
NCBI Official Full Name
syntaxin-binding protein 1
NCBI Official Synonym Full Names
syntaxin binding protein 1
NCBI Official Symbol
Stxbp1
NCBI Official Synonym Symbols
Sec1; nSec1; NSEC1A; n-sec1; rbSec1; ANC18HA; Unc18-1; rbSec1A; rbSec1B; Munc18-1
NCBI Protein Information
syntaxin-binding protein 1; p67; protein unc-18 homolog 1; protein unc-18 homolog A; unc-18-1; unc-18A
UniProt Protein Name
Syntaxin-binding protein 1
UniProt Gene Name
Stxbp1
UniProt Synonym Gene Names
Unc18a; Unc18-1; Unc-18A
UniProt Entry Name
STXB1_RAT
NCBI Description
inhibits binding of synaptobrevin and SNAP-25 to syntaxin 1A to modulate SNARE complex formation; acts to regulate neurotransmission [RGD, Feb 2006]
Uniprot Description
STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.
Protein type: Vesicle
Cellular Component: nucleoplasm; protein complex; mitochondrion; cell; cytoplasm; plasma membrane; terminal button; cytosol; myelin sheath
Molecular Function: protein domain specific binding; identical protein binding; protein binding; SNARE binding; syntaxin binding; syntaxin-1 binding; protein N-terminus binding; protein kinase binding
Biological Process: exocytosis; protein stabilization; synaptic vesicle maturation; neurotransmitter secretion; positive regulation of calcium ion-dependent exocytosis; protein transport; platelet degranulation; axon target recognition; neuromuscular synaptic transmission; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; vesicle docking during exocytosis; positive regulation of exocytosis
Research Articles on MUNC-18
Similar Products
Product Notes
The MUNC-18 stxbp1 (Catalog #AAA221521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI MUNC-18 (pSer515) reacts with Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence), Xenopus (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's MUNC-18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB)*. Western Blot: This item detects a band/s of approximately 65kDa in rat cortex cell lysates Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. Researchers should empirically determine the suitability of the MUNC-18 stxbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUNC-18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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