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Western Blot (WB) (Western blot analysis of MUC16 using NIH-3T3 whole lysates.)

Rabbit anti-Human MUC16 Polyclonal Antibody | anti-MUC16 antibody

MUC16 Antibody

Gene Names
MUC16; CA125
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MUC16; Polyclonal Antibody; MUC16 Antibody; CA 125; CA-125; CA125; CA125 ovarian cancer antigen; Cancer antigen 125; FLJ14303; MUC 16; MUC-16; MUC16_HUMAN; Mucin 16; Mucin 16 cell surface associated; Mucin-16; Mucin16; Ovarian cancer related tumor marker CA125; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125; anti-MUC16 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
MUC16 antibody detects endogenous levels of total MUC16
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
14507
Applicable Applications for anti-MUC16 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human MUC16
Subcellular Location
Cell Membrane. Secreted > Extracellular Space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Tissue Specificity
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of MUC16 using NIH-3T3 whole lysates.)

Western Blot (WB) (Western blot analysis of MUC16 using NIH-3T3 whole lysates.)
Related Product Information for anti-MUC16 antibody
Function: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Subunit Structure: Binds to MSLN. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Post-translational Modifications: Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
Similarity: Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing between 12 and 60 partially conserved tandem repeats of 156 amino acids and a C-terminal transmembrane contain domain with a short cytoplasmic tail.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 2353 kDa
Predicted: 2 kDa
NCBI Official Full Name
mucin-16
NCBI Official Synonym Full Names
mucin 16, cell surface associated
NCBI Official Symbol
MUC16
NCBI Official Synonym Symbols
CA125
NCBI Protein Information
mucin-16
UniProt Protein Name
Mucin-16
Protein Family
UniProt Gene Name
MUC16
UniProt Synonym Gene Names
CA-1251 PublicationManual assertion based on opinion iniRef.2

NCBI Description

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes. [provided by RefSeq, May 2017]

Uniprot Description

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Research Articles on MUC16

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Product Notes

The MUC16 muc16 (Catalog #AAA9608483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MUC16 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUC16 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the MUC16 muc16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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