Typical Testing Data/Standard Curve (for reference only)
Human MUC16 ELISA Kit | MUC16 elisa kit
Human MUC16 ELISA Kit
Gene Names
MUC16; CA125
Reactivity
Human
Synonyms
MUC16; Human MUC16 ELISA Kit; CA125; Mucin-16; MUC-16; Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125; MUC16 elisa kit
Reactivity
Human
Specificity
The Human MUC16 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human MUC16 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
14507
Samples
Cell culture supernates, serum, plasma, saliva, urine, human milk
Sensitivity
< 10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUC16 elisa kit
Principle of the Assay: The MyBioSource' Human MUC16 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MUC16 in Cell Culture Supernates, Serum, Plasma, Saliva, Urine, Human Milk. This assay employs an antibody specific for Human MUC16 coated on a 96-well plate. Standards and samples are pipetted into the wells and MUC16 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MUC16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MUC16 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.
Background/Introduction: CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
1,519,175 Da
NCBI Official Full Name
mucin-16
NCBI Official Synonym Full Names
mucin 16, cell surface associated
NCBI Official Symbol
MUC16
NCBI Official Synonym Symbols
CA125
NCBI Protein Information
mucin-16
UniProt Protein Name
Mucin-16
Protein Family
UniProt Gene Name
MUC16
UniProt Synonym Gene Names
CA-125
UniProt Entry Name
MUC16_HUMAN
Uniprot Description
MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Protein type: Cell adhesion; Membrane protein, integral; Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extrinsic to membrane; Golgi lumen; vesicle
Molecular Function: protein binding
Biological Process: O-glycan processing
Research Articles on MUC16
Similar Products
Product Notes
The Human MUC16 muc16 (Catalog #AAA8248378) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8248378 ELISA Kit recognizes Human MUC16. It is sometimes possible for the material contained within the vial of "MUC16, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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