Rabbit MTOR Polyclonal Antibody | anti-MTOR antibody

Rabbit Polyclonal (IgG) to Human MTOR

Cat. Num. AAA242903

• Gene Names: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MTOR; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human MTOR; Anti-MTOR Antibody (aa2447-2496) IHC-plus; FRAP1; FRAP2; FRAP; Mammalian target of rapamycin; RAFT1; Rapamycin and FKBP12 target 1; RAPT1; Rapamycin target protein 1; Human MTOR; anti-MTOR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: mTOR Antibody detects endogenous levels of total mTOR protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2549

• Applicable Applications for anti-MTOR antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human mTOR (aa2447-2496).
• Immunogen: MTOR antibody was raised against synthetic peptide from human mTOR (aa2447-2496).
• Antigen Modification: aa2447-2496
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-MTOR antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of extracts from A549 cells, using mTOR Antibody. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-MTOR antibody

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin.

Product Categories/Family for anti-MTOR antibody

Family: Protein Kinase; Subfamily: PI3/PI4

NCBI and Uniprot Product Information

• NCBI GI #: 4826730
• NCBI GeneID: 2475
• NCBI Accession #: NP_004949.1
• NCBI GenBank Nucleotide #: NM_004958.3
• UniProt Accession #: P42345; Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3
• Molecular Weight: 288,892 Da
• NCBI Official Full Name: serine/threonine-protein kinase mTOR
• NCBI Official Synonym Full Names: mechanistic target of rapamycin (serine/threonine kinase)
• NCBI Official Symbol: MTOR
• NCBI Official Synonym Symbols: FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• NCBI Protein Information: serine/threonine-protein kinase mTOR; rapamycin target protein 1; mammalian target of rapamycin; rapamycin and FKBP12 target 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; FKBP12-rapamycin complex-associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1
• UniProt Protein Name: Serine/threonine-protein kinase mTOR
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: MTOR
• UniProt Synonym Gene Names: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
• UniProt Entry Name: MTOR_HUMAN

NCBI Description

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: Motility/polarity/chemotaxis; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; EC 2.7.11.1; Kinase, protein; Autophagy; ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: endoplasmic reticulum membrane; PML body; lysosome; lysosomal membrane; dendrite; endomembrane system; cytosol; nucleoplasm; Golgi membrane; mitochondrial outer membrane; membrane; cell soma; cytoplasm; TORC2 complex; phosphoinositide 3-kinase complex; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; ribosome binding; phosphoprotein binding; drug binding; kinase activity; ATP binding

Biological Process: negative regulation of autophagy; TOR signaling pathway; regulation of myelination; nerve growth factor receptor signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; negative regulation of NFAT protein import into nucleus; response to amino acid stimulus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; ruffle organization and biogenesis; positive regulation of transcription from RNA polymerase III promoter; innate immune response; regulation of fatty acid beta-oxidation; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; regulation of protein kinase activity; phosphorylation; growth