Rabbit anti-Human MTOR Polyclonal Antibody | anti-MTOR antibody

Rabbit Polyclonal to Human MTOR

Cat. Num. AAA240649

• Gene Names: MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MTOR; Polyclonal Antibody; Rabbit Polyclonal to Human MTOR; Anti-MTOR Antibody (phospho-Ser2448) IHC-plus; FRAP1; FRAP2; FRAP; Mammalian target of rapamycin; RAFT1; Rapamycin and FKBP12 target 1; RAPT1; Rapamycin target protein 1; Human MTOR; anti-MTOR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Amino acids 2440-2457 of human mTOR.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 2549

• Applicable Applications for anti-MTOR antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:15000 - 1:65000), IHC-P (5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human mTOR.
• Immunogen Type: Synthetic peptide
• Immunogen: MTOR antibody was raised against synthetic peptide from human mTOR.
• Antigen Modification: pSer2448
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MTOR antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-mTOR pS2448 Antibody - Western Blot. Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250 kD band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.)

Related Product Information for anti-MTOR antibody

The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin.

Product Categories/Family for anti-MTOR antibody

Family: Protein Kinase; Subfamily: PI3/PI4

NCBI and Uniprot Product Information

• NCBI GI #: 4826730
• NCBI GeneID: 2475
• NCBI Accession #: NP_004949.1
• NCBI GenBank Nucleotide #: NM_004958.3
• UniProt Accession #: P42345; Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3
• Molecular Weight: 288,892 Da
• NCBI Official Full Name: serine/threonine-protein kinase mTOR
• NCBI Official Synonym Full Names: mechanistic target of rapamycin (serine/threonine kinase)
• NCBI Official Symbol: MTOR
• NCBI Official Synonym Symbols: FRAP; FRAP1; FRAP2; RAFT1; RAPT1
• NCBI Protein Information: serine/threonine-protein kinase mTOR; rapamycin target protein 1; mammalian target of rapamycin; rapamycin and FKBP12 target 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; FKBP12-rapamycin complex-associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1
• UniProt Protein Name: Serine/threonine-protein kinase mTOR
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: MTOR
• UniProt Synonym Gene Names: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
• UniProt Entry Name: MTOR_HUMAN

NCBI Description

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Autophagy; Protein kinase, atypical; ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: endoplasmic reticulum membrane; PML body; lysosome; lysosomal membrane; dendrite; endomembrane system; cytosol; nucleoplasm; Golgi membrane; mitochondrial outer membrane; cell soma; membrane; cytoplasm; TORC2 complex; phosphoinositide 3-kinase complex; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; ribosome binding; phosphoprotein binding; drug binding; kinase activity; ATP binding

Biological Process: negative regulation of autophagy; regulation of myelination; TOR signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; response to amino acid stimulus; negative regulation of NFAT protein import into nucleus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; ruffle organization and biogenesis; insulin receptor signaling pathway; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; regulation of protein kinase activity; phosphorylation; growth