Chicken Vascular endothelial growth factor receptor 1 ELISA Kit | FLT1 elisa kit

Chicken Vascular endothelial growth factor receptor 1 ELISA Kit

Cat. Num. AAA288891

• Reactivity: Chicken
• Synonyms: Vascular endothelial growth factor receptor 1; Chicken Vascular endothelial growth factor receptor 1 ELISA Kit; VEGFR-1; FLT1; VEGFR1; 2.7.10.1; FLT1 elisa kit

• Reactivity: Chicken
• Specificity: Recombinant and natural Chicken VEGFR-1
• Sequence Length: 1327

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.78-50 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=6.9%
• Inter-assay Precision: Inter-Assay CV: <=10.3%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLT1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45383624
• NCBI GeneID: 374100
• NCBI Accession #: NP_989583.1
• NCBI GenBank Nucleotide #: NM_204252.1
• UniProt Accession #: Q8QHL3; Q8QHL2
• Molecular Weight: 76,096 Da
• NCBI Official Full Name: vascular endothelial growth factor receptor 1
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 1
• NCBI Official Symbol: FLT1
• NCBI Protein Information: vascular endothelial growth factor receptor 1
• UniProt Protein Name: Vascular endothelial growth factor receptor 1
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: FLT1
• UniProt Synonym Gene Names: VEGFR1; VEGFR-1
• UniProt Entry Name: VGFR1_CHICK

Uniprot Description

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the regulation of angiogenesis, cell survival, cell migration, macrophage function, and chemotaxis. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates PLCG1. Promotes phosphorylation of AKT1 and CBL ().