Goat MTHFD1 Polyclonal Antibody | anti-MTHFD1 antibody

Goat Polyclonal to Human MTHFD1

Cat. Num. AAA244209

• Gene Names: MTHFD1; MTHFC; MTHFD
• Reactivity: Bat, Gibbon, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit; Predicted Reactivity: Rat, Dog, Chicken, Xenopus (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MTHFD1; Polyclonal Antibody; Goat Polyclonal to Human MTHFD1; Anti-MTHFD1 Antibody (Internal) IHC-plus; C1-THF synthase; MTHFC; MTHFD; Human MTHFD1; anti-MTHFD1 antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit; Predicted Reactivity: Rat, Dog, Chicken, Xenopus (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human MTHFD1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 935

• Applicable Applications for anti-MTHFD1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:1000), IHC-P (3.75 ug/ml), WB (1 - 3 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide RGDLNDCFIPCTPK from an internal region of human MTHFD1 (NP_005947.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: MTHFD1 antibody was raised against synthetic peptide RGDLNDCFIPCTPK from an internal region of human MTHFD1 (NP_005947.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Pig (100%); Rat, Dog, Opossum, Turkey, Chicken, Lizard, Xenopus (93%); Bovine, Stickleback, Pufferfish, Zebrafish (86%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MTHFD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody (1 ug/ml) staining of Human Substantia Nigra lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 222136639
• NCBI GeneID: 4522
• NCBI Accession #: NP_005947.3
• NCBI GenBank Nucleotide #: NM_005956.3
• UniProt Accession #: P11586; Q86VC9; Q9BVP5; B2R5Y2; G3V2B8
• Molecular Weight: 101,559 Da
• NCBI Official Full Name: C-1-tetrahydrofolate synthase, cytoplasmic
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• NCBI Official Symbol: MTHFD1
• NCBI Official Synonym Symbols: MTHFC; MTHFD
• NCBI Protein Information: C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase; 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase
• UniProt Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Gene Name: MTHFD1
• UniProt Synonym Gene Names: MTHFC; MTHFD; C1-THF synthase
• UniProt Entry Name: C1TC_HUMAN

NCBI Description

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]

Uniprot Description

MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.

Protein type: EC 1.5.1.5; Oxidoreductase; Ligase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 6.3.4.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Hydrolase; EC 3.5.4.9

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: mitochondrion; membrane; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity

Biological Process: histidine biosynthetic process; folic acid and derivative biosynthetic process; vitamin metabolic process; methionine biosynthetic process; purine nucleotide biosynthetic process; folic acid metabolic process; one-carbon compound metabolic process; water-soluble vitamin metabolic process

Disease: Neural Tube Defects, Folate-sensitive