Rabbit anti-Human MTHFD1 Polyclonal Antibody | anti-MTHFD1 antibody

Anti-MTHFD1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102062

• Gene Names: MTHFD1; MTHFC; MTHFD
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MTHFD1; Polyclonal Antibody; Anti-MTHFD1 Antibody; Rabbit Polyclonal; MTHFD1 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-MTHFD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MTHFD1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MTHFD1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human MTHFD1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MTHFD1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human MTHFD1 in human lung cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of MTHFD1 in 293 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MTHFD1 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)

Related Product Information for anti-MTHFD1 antibody

The methylenetetrahydrofolate dehydrogenase (MTHFD1) gene, as one of the key genes involved in the folate pathway, has been reported to play a critical role in the pathogenesis of neural tube defects (NTDs). Methylenetetrahydrofolate dehydrogenase (MTHFD1) deficiency has recently been reported to cause a folate-responsive syndrome displaying a phenotype that includes megaloblastic anemia and severe combined immunodeficiency. Methylenetetrahydrofolate dehydrogenase (MTHFD1) catalyzes three sequential reactions that metabolize derivatives of tetrahydrofolate (THF) in folate-dependent one-carbon metabolism. Impaired MTHFD1 flux has been linked to disturbed lipid metabolism and oxidative stress.

NCBI and Uniprot Product Information

• NCBI GI #: 222136639
• NCBI GeneID: 4522
• NCBI Accession #: NP_005947.3
• NCBI GenBank Nucleotide #: NM_005956.3
• UniProt Accession #: P11586; Q86VC9; Q9BVP5; B2R5Y2; G3V2B8
• Molecular Weight: 101,559 Da
• NCBI Official Full Name: C-1-tetrahydrofolate synthase, cytoplasmic
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
• NCBI Official Symbol: MTHFD1
• NCBI Official Synonym Symbols: MTHFC; MTHFD
• NCBI Protein Information: C-1-tetrahydrofolate synthase, cytoplasmic; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase; 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase
• UniProt Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Gene Name: MTHFD1
• UniProt Synonym Gene Names: MTHFC; MTHFD; C1-THF synthase
• UniProt Entry Name: C1TC_HUMAN

NCBI Description

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]

Uniprot Description

MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.

Protein type: EC 1.5.1.5; Oxidoreductase; Ligase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 6.3.4.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Hydrolase; EC 3.5.4.9

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: mitochondrion; membrane; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity

Biological Process: histidine biosynthetic process; folic acid and derivative biosynthetic process; vitamin metabolic process; methionine biosynthetic process; purine nucleotide biosynthetic process; folic acid metabolic process; one-carbon compound metabolic process; water-soluble vitamin metabolic process

Disease: Neural Tube Defects, Folate-sensitive