Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit MTHFD1 Polyclonal Antibody | anti-MTHFD1 antibody
MTHFD1 Antibody (Center P550)
WB~~1:1000
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MTHFD1 Antibody (Center P550) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(Western blot analysis of MTHFD1 Antibody (Center P550) in Y79 cell line lysates (35ug/lane). MTHFD1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MTHFD1 Antibody (Center P550) in Y79 cell line lysates (35ug/lane). MTHFD1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(MTHFD1 Antibody (Center P550) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(MTHFD1 Antibody (Center P550) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MTHFD1 Antibody (Center P550) for immunohistochemistry. Clinical relevance has not been evaluated.)
Schmidt A., Biochemistry 39:6325-6335(2000)
Hol F.A., Clin. Genet. 53:119-125(1998)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]
Uniprot Description
MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.
Protein type: EC 3.5.4.9; Hydrolase; Oxidoreductase; Ligase; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 6.3.4.3; EC 1.5.1.5; Cofactor and Vitamin Metabolism - one carbon pool by folate
Chromosomal Location of Human Ortholog: 14q24
Cellular Component: mitochondrion; membrane; cytosol
Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity
Biological Process: histidine biosynthetic process; folic acid and derivative biosynthetic process; vitamin metabolic process; methionine biosynthetic process; folic acid metabolic process; purine nucleotide biosynthetic process; one-carbon compound metabolic process; water-soluble vitamin metabolic process
Disease: Neural Tube Defects, Folate-sensitive
Research Articles on MTHFD1
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Product Notes
The MTHFD1 mthfd1 (Catalog #AAA9203969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 535-562. The MTHFD1 Antibody (Center P550) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's MTHFD1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). IF~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the MTHFD1 mthfd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTHFD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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