Rabbit MTHFD1 Polyclonal Antibody | anti-MTHFD1 antibody

MTHFD1 Antibody

Cat. Num. AAA9611909

• Gene Names: MTHFD1; CIMAH; MTHFC; MTHFD
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MTHFD1; Polyclonal Antibody; MTHFD1 Antibody; 5; 10 methylenetetrahydrofolate dehydrogenase 5; 10 methylenetetrahydrofolate cyclohydrolase; 10 formyltetrahydrofolate synthetase; C 1 tetrahydrofolate synthase cytoplasmic; C1 tetrahydrofolate synthase cytoplasmic; C1 THF synthase; C1-THF synthase; C1TC; C1TC_HUMAN; Cytoplasmic C 1 tetrahydrofolate synthase; Formyltetrahydrofolate synthetase; Methenyltetrahydrofolate cyclohydrolase; Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase; Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase; Methylenetetrahydrofolate dehydrogenase; MTHFC; MTHFD; NADP dependent cyclohydrolase formyltetrahydrofolate synthetase; OTTHUMP00000180657; anti-MTHFD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MTHFD1 Antibody detects endogenous levels of MTHFD1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 935

• Applicable Applications for anti-MTHFD1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human MTHFD1.
• Tissue Specificity: Ubiquitous.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HEK-293 cells, using MTHFD1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MTHFD1 antibody

Description: 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.

Subunit Structure: Homodimer.

Similarity: This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

NCBI and Uniprot Product Information

• NCBI GI #: 222136639
• NCBI GeneID: 4522
• NCBI Accession #: NP_005947.3
• NCBI GenBank Nucleotide #: NP_005947.3
• UniProt Accession #: P11586
• Molecular Weight: Observed: 101 kDa; Predicted: 102 kDa
• NCBI Official Full Name: C-1-tetrahydrofolate synthase, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
• NCBI Official Symbol: MTHFD1
• NCBI Official Synonym Symbols: CIMAH; MTHFC; MTHFD
• NCBI Protein Information: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Protein Name: C-1-tetrahydrofolate synthase, cytoplasmic
• UniProt Gene Name: MTHFD1
• UniProt Synonym Gene Names: MTHFC; MTHFD; C1-THF synthase
• UniProt Entry Name: C1TC_HUMAN

NCBI Description

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008]

Uniprot Description

MTHFD1: an ubiquitous trifunctional enzyme containing two major domains: a N-terminal part, containing methylene-THF dehydrogenase and methenyl-THF cyclohydrolase activities, and a C-terminal part containing a formyl-THF synthetase domain.

Protein type: EC 1.5.1.5; Oxidoreductase; Ligase; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 6.3.4.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Hydrolase; EC 3.5.4.9

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: mitochondrion; membrane; cytosol

Molecular Function: methylenetetrahydrofolate dehydrogenase (NADP+) activity; formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; ATP binding; methylenetetrahydrofolate dehydrogenase activity

Biological Process: histidine biosynthetic process; folic acid and derivative biosynthetic process; vitamin metabolic process; methionine biosynthetic process; purine nucleotide biosynthetic process; folic acid metabolic process; one-carbon compound metabolic process; water-soluble vitamin metabolic process

Disease: Neural Tube Defects, Folate-sensitive