Rabbit anti-Human MTF2 Polyclonal Antibody | anti-MTF2 antibody

MTF2, ID (MTF2, PCL2, Metal-response element-binding transcription factor 2, Metal regulatory transcription factor 2, Metal-response element DNA-binding protein M96, Polycomb-like protein 2) (Azide free) (HRP)

Cat. Num. AAA6322707

• Gene Names: MTF2; M96; PCL2; TDRD19A; dJ976O13.2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MTF2; Polyclonal Antibody; ID (MTF2; PCL2; Metal-response element-binding transcription factor 2; Metal regulatory transcription factor 2; Metal-response element DNA-binding protein M96; Polycomb-like protein 2) (Azide free) (HRP); Polycomb-like protein 2; anti-MTF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MTF2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MTF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the Central region of human MTF2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MTF2 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MTF2 antibody detected the MTF2 protein (arrow).)

Related Product Information for anti-MTF2 antibody

MTF2 binds to the metal-regulating-element (MRE) of metallothionein-1A gene promoter. Binding is zinc-dependent (By similarity).

Product Categories/Family for anti-MTF2 antibody

Antibodies; Transcription Factors

References

Wang, S., et al. Gene 343(1):69-78(2004). Colland, F., et al. Genome Res. 14(7):1324-1332(2004). Inouye, C., et al. DNA Cell Biol. 13(7):731-742(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 256419012
• NCBI GeneID: 22823
• NCBI Accession #: NP_001157863.1
• NCBI GenBank Nucleotide #: NM_001164391.1
• UniProt Accession #: Q9Y483; Q9UES9; Q9UP40; A6NGQ9; A8K2Q3; B1AKT5; B1AKT6
• Molecular Weight: 67,106 Da
• NCBI Official Full Name: metal-response element-binding transcription factor 2 isoform c
• NCBI Official Synonym Full Names: metal response element binding transcription factor 2
• NCBI Official Symbol: MTF2
• NCBI Official Synonym Symbols: M96; PCL2; TDRD19A; dJ976O13.2
• NCBI Protein Information: metal-response element-binding transcription factor 2; hPCl2; polycomb-like 2; polycomb-like protein 2; tudor domain containing 19A; putative DNA binding protein; metal regulatory transcription factor 2; metal-response element DNA-binding protein M96; met
• UniProt Protein Name: Metal-response element-binding transcription factor 2
• Protein Family: Mitochondrial transcription factor
• UniProt Gene Name: MTF2
• UniProt Synonym Gene Names: PCL2; hPCl2
• UniProt Entry Name: MTF2_HUMAN

Uniprot Description

MTF2: Binds to the metal-regulating-element (MRE) of metallothionein-1A gene promoter. Binding is zinc-dependent. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: nucleoplasm; ESC/E(Z) complex; cytoplasm; nucleus

Molecular Function: DNA binding; zinc ion binding; methylated histone residue binding

Biological Process: segment specification; negative regulation of gene expression, epigenetic; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; gene expression; stem cell differentiation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic