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Western Blot (WB) (Western blot analysis of lysate from mouse cerebellum tissue lysate, using MTF2 Antibody (Center). MBS9205104 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

Rabbit MTF2 Polyclonal Antibody | anti-MTF2 antibody

MTF2 Antibody (Center)

Gene Names
MTF2; M96; PCL2; TDRD19A; dJ976O13.2
Reactivity
Human, mouse, rat
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MTF2; Polyclonal Antibody; MTF2 Antibody (Center); Metal-response element-binding transcription factor 2; Metal regulatory transcription factor 2; Metal-response element DNA-binding protein M96; Polycomb-like protein 2; hPCl2; PCL2; anti-MTF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MTF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-317 amino acids from the Central region of human MTF2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
289-317
Sequence Length
491
Applicable Applications for anti-MTF2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from mouse cerebellum tissue lysate, using MTF2 Antibody (Center). MBS9205104 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB) (Western blot analysis of lysate from mouse cerebellum tissue lysate, using MTF2 Antibody (Center). MBS9205104 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB)

(Western blot analysis of lysates from CEM, KG-1 cell line, mouse cerebellum and rat brain tissue lysate (from left to right), using MTF2 Antibody (Center). MBS9205104 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from CEM, KG-1 cell line, mouse cerebellum and rat brain tissue lysate (from left to right), using MTF2 Antibody (Center). MBS9205104 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Western Blot (WB)

(MTF2 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MTF2 antibody detected the MTF2 protein (arrow).)

Western Blot (WB) (MTF2 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MTF2 antibody detected the MTF2 protein (arrow).)
Related Product Information for anti-MTF2 antibody
MTF2 binds to the metal-regulating-element (MRE) of metallothionein-1A gene promoter. Binding is zinc-dependent (By similarity).
References
Wang, S., et al. Gene 343(1):69-78(2004)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Inouye, C., et al. DNA Cell Biol. 13(7):731-742(1994)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67106
NCBI Official Full Name
metal-response element-binding transcription factor 2 isoform c
NCBI Official Synonym Full Names
metal response element binding transcription factor 2
NCBI Official Symbol
MTF2
NCBI Official Synonym Symbols
M96; PCL2; TDRD19A; dJ976O13.2
NCBI Protein Information
metal-response element-binding transcription factor 2
UniProt Protein Name
Metal-response element-binding transcription factor 2
UniProt Gene Name
MTF2
UniProt Synonym Gene Names
PCL2; hPCl2
UniProt Entry Name
MTF2_HUMAN

Uniprot Description

MTF2: Binds to the metal-regulating-element (MRE) of metallothionein-1A gene promoter. Binding is zinc-dependent. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: nucleoplasm; ESC/E(Z) complex; cytoplasm; nucleus

Molecular Function: DNA binding; zinc ion binding; methylated histone residue binding

Biological Process: segment specification; negative regulation of gene expression, epigenetic; stem cell maintenance; positive regulation of transcription from RNA polymerase II promoter; gene expression; chromatin modification; stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic

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Product Notes

The MTF2 mtf2 (Catalog #AAA9205104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 289-317. The MTF2 Antibody (Center) reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's MTF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the MTF2 mtf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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