Western Blot (WB)
(Figure 1. Western blot analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (MBS1753732).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A431 whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: rat RH35 whole cell lysatesLane 5: mouse liver tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-MT2-MMP/MMP15 antigen affinity purified polyclonal antibody (Catalog # MBS1753732) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for MT2-MMP/MMP15 at approximately 74KD. The expected band size for MT2-MMP/MMP15 is at 74KD. )
Rabbit MT2-MMP/MMP15 Polyclonal Antibody | anti-MMP15 antibody
Anti-MT2-MMP/MMP15 Antibody
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (MBS1753732).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A431 whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: rat RH35 whole cell lysatesLane 5: mouse liver tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-MT2-MMP/MMP15 antigen affinity purified polyclonal antibody (Catalog # MBS1753732) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for MT2-MMP/MMP15 at approximately 74KD. The expected band size for MT2-MMP/MMP15 is at 74KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of MT2-MMP/MMP15 using anti-MT2-MMP/MMP15 antibody (MBS1753732).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A431 whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: rat RH35 whole cell lysatesLane 5: mouse liver tissue lysatesLane 6: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-MT2-MMP/MMP15 antigen affinity purified polyclonal antibody (Catalog # MBS1753732) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for MT2-MMP/MMP15 at approximately 74KD. The expected band size for MT2-MMP/MMP15 is at 74KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of MT2-MMP/MMP15 using anti- MT2-MMP/MMP15 antibody (MBS1753732).MT2-MMP/MMP15 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MT2-MMP/MMP15 Antibody (MBS1753732) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of MT2-MMP/MMP15 using anti- MT2-MMP/MMP15 antibody (MBS1753732).MT2-MMP/MMP15 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MT2-MMP/MMP15 Antibody (MBS1753732) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-MT2-MMP/MMP15 antibody (MBS1753732).Overlay histogram showing CACO-2 cells stained with MBS1753732 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT2-MMP/MMP15 Antibody (MBS1753732, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-MT2-MMP/MMP15 antibody (MBS1753732).Overlay histogram showing CACO-2 cells stained with MBS1753732 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT2-MMP/MMP15 Antibody (MBS1753732, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Sato, H., Tanaka, M., Takino, T., Inoue, M., Seiki, M. Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12. 2, 16q12. 2-q21, and 8q21, respectively, by in situ hybridization. Genomics 39: 412-413, 1997.
3. Takino, T., Sato, H., Shinagawa, A., Seiki, M. Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library: MT-MMPs form a unique membrane-type subclass in the MMP family. J. Biol. Chem. 270: 23013-23020, 1995.
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.
Protein type: EC 3.4.24.-; Protease; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16q13
Cellular Component: extracellular matrix; integral to plasma membrane; plasma membrane
Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding
Biological Process: positive regulation of catalytic activity; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; protein modification process; proteolysis; response to estradiol stimulus
Research Articles on MMP15
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Product Notes
The MMP15 mmp15 (Catalog #AAA1753732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MT2-MMP/MMP15 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MT2-MMP/MMP15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the MMP15 mmp15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MT2-MMP/MMP15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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