Rabbit anti-Human SYNE3 Polyclonal Antibody | anti-SYNE3 antibody

SYNE3 Polyclonal Antibody

Cat. Num. AAA3010112

• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SYNE3; Polyclonal Antibody; SYNE3 Polyclonal Antibody; C14orf139; C14orf49; KASH3; LINC00341; NCRNA00341; NET53; Nesp3; Nesprin3; Nesprin 3; Nesprin-3; anti-SYNE3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SYNE3 polyclonal antibody detects endogenous levels of SYNE3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 975

• Applicable Applications for anti-SYNE3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant Protein of human SYNE3
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using SYNE3 antibody at 1:3000 dilution.)

Related Product Information for anti-SYNE3 antibody

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 145580592
• NCBI Accession #: NP_689805.3
• NCBI GenBank Nucleotide #: NP_689805.3
• UniProt Accession #: Q6ZMZ3
• Molecular Weight: 112kDa
• NCBI Official Full Name: nesprin-3 isoform 1
• UniProt Protein Name: Nesprin-3
• Protein Family: Nesprin
• UniProt Gene Name: SYNE3
• UniProt Synonym Gene Names: C14orf49
• UniProt Entry Name: SYNE3_HUMAN

Uniprot Description

SYNE3: Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow- induced centrosome polarization and directional migration in aortic endothelial cells. Belongs to the nesprin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: nuclear outer membrane; rough endoplasmic reticulum; membrane; integral to membrane; nuclear envelope

Molecular Function: actin filament binding; protein binding

Biological Process: regulation of cell shape; cytoskeleton organization and biogenesis