Human carbonic anhydrase III, muscle specific ELISA Kit | CA3 elisa kit

Human Carbonic Anhydrase 3, CA3 ELISA Kit

Cat. Num. AAA705713

• Gene Names: CA3; Car3; CAIII
• Reactivity: Human
• Synonyms: carbonic anhydrase III; muscle specific; Human Carbonic Anhydrase 3; CA3 ELISA Kit; Human Carbonic Anhydrase 3 (CA3) ELISA Kit; CAIII; Car3; CA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CA3. No significant cross-reactivity or interference between human CA3 and analogues was observed.
• Sequence Length: 260

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 7.8 ng/ml-500 ng/ml
• Sensitivity: 1.95 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4885099
• NCBI GeneID: 761
• NCBI Accession #: NP_005172.1
• NCBI GenBank Nucleotide #: NM_005181.3
• UniProt Accession #: P07451; O60842; B2R867; B3KUC8
• Molecular Weight: 29,557 Da
• NCBI Official Full Name: carbonic anhydrase 3
• NCBI Official Synonym Full Names: carbonic anhydrase III, muscle specific
• NCBI Official Symbol: CA3
• NCBI Official Synonym Symbols: Car3; CAIII
• NCBI Protein Information: carbonic anhydrase 3; CA-III; HEL-S-167mP; carbonate dehydratase III; epididymis secretory sperm binding protein Li 167mP
• UniProt Protein Name: Carbonic anhydrase 3
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA3
• UniProt Synonym Gene Names: CA-III
• UniProt Entry Name: CAH3_HUMAN

NCBI Description

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Reversible hydration of carbon dioxide.

Catalytic activity: H2CO3 = CO2 + H2O.

Cofactor: Zinc.

Enzyme regulation: Activated by proton donors such as imidazole and the dipeptide histidylhistidine. Inhibited by coumarins and sulfonamide derivatives such as acetazolamide.3 PublicationsManual assertion based on experiment in:Ref.8