Rabbit MSY2 Polyclonal Antibody | anti-MSY2 antibody

MSY2 (G311) Polyclonal Antibody

Cat. Num. AAA3002228

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MSY2; Polyclonal Antibody; MSY2 (G311) Polyclonal Antibody; Y-box-binding protein 2; Contrin; DNA-binding protein C; Dbpc; Germ cell-specific Y-box-binding protein; MSY2 homolog; YBX2; CSDA3; anti-MSY2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MSY2 (G311) polyclonal antibody detects endogenous levels of MSY2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 364

• Applicable Applications for anti-MSY2 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 287-325 of Human MSY2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MSY2 (G311) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-MSY2 antibody

Contrin (MSY2 in mouse) and YB-2 (MSY3/4 in mouse) belong to the Y-box family of multifunctional proteins that regulate both transcription and translation.Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing arginine clusters and aromatic residues. MSY2 (Contrin) is a expressed in testis and ovary, where it may repress translation of parental mRNA. The gene encoding human Contrin maps to chromosome 17p11.2-13.1. MSY3/4 (YB-2) is also known as DNA binding protein A and is highly expressed in testis, heart and muscle. Contrin and YB-2 bind to the consensus sequence 5'-UCCAUCA-3' contained in the Y-box element.

NCBI and Uniprot Product Information

• NCBI GI #: 156415990
• NCBI Accession #: NP_057066.2
• NCBI GenBank Nucleotide #: NP_057066.2
• UniProt Accession #: Q9Y2T7
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: Y-box-binding protein 2
• UniProt Protein Name: Y-box-binding protein 2
• UniProt Gene Name: YBX2
• UniProt Synonym Gene Names: CSDA3; MSY2; Dbpc
• UniProt Entry Name: YBOX2_HUMAN

Uniprot Description

YBX2: Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: polysome; cytoplasm; nucleolus; nucleus

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding; DNA binding; translation regulator activity; chromatin binding; lipid binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; translational attenuation; mRNA stabilization; negative regulation of translation; spermatogenesis; negative regulation of binding; oocyte development; spermatid development