Rabbit YBOX1/YB-1 Polyclonal Antibody | anti-YBOX1/YB-1 antibody

YBOX1/YB-1 Antibody

Cat. Num. AAA9435103

• Gene Names: YBX2; DBPC; MSY2; CSDA3; CONTRIN
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide affinity purified
• Synonyms: YBOX1/YB-1; Polyclonal Antibody; YBOX1/YB-1 Antibody; BP 8 antibody; CBF-A antibody; CCAAT binding transcription factor I subunit A antibody; CCAAT-binding transcription factor I subunit A antibody; CSDA2 antibody; CSDB antibody; DBPB antibody; DNA binding protein B antibody; DNA-binding protein B antibody; EFI-A antibody; Enhancer factor I subunit A antibody; MDR NF1 antibody; MGC104858 antibody; MGC110976 antibody; MGC117250 antibody; NSEP 1 antibody; NSEP1 antibody; Nuclease sensitive element binding protein 1 antibody; Nuclease-sensitive element-binding protein 1 antibody; p50 antibody; Q15905 antibody; Y-box binding protein 1 antibody; Y-box transcription factor antibody; Y-box-binding protein 1 antibody; YB 1 antibody; YB-1 antibody; YBOX1_HUMAN antibody; YBX 1 antibody; ybx1 antibody; anti-YBOX1/YB-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Peptide affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-YBOX1/YB-1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Peptide
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis on mouse testis lysates using anti-YB1 rabbit polyclonal antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-YB1 rabbit polyclonal antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded mouse ovary tissue using anti-YB1 rabbit polyclonal antibody.)

Immunocytochemistry (ICC)

(Immunocytochemical staining of Hela cells using anti-YB1 rabbit polyclonal antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with YB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-YBOX1/YB-1 antibody

MSY2 and YB-2 (MSY3, 4) belong to the Y-box family of multifunctional proteins that regulate both transcription and translation. Y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins. The modular structure of Y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing arginine clusters and aromatic residues. MSY2 is expressed in testis and ovary where it may repress translation of parental mRNA. The gene encoding human MSY2 maps to chromosome 17p11.2-13.1. YB-2 (MSY3, 4 in mouse) is also known as DNA binding protein A and is highly expressed in the testis, heart and muscle. MSY2 and YB-2 bind to the consensus sequence 5'-UCCAUCA-3' contained in the Y-box element.

Product Categories/Family for anti-YBOX1/YB-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 156415990
• NCBI GeneID: 51087
• NCBI Accession #: NP_057066.2
• NCBI GenBank Nucleotide #: NM_015982.3
• UniProt Accession #: Q9Y2T7; Q8N4P0; D3DTP1
• Molecular Weight: 38,518 Da
• NCBI Official Full Name: Y-box-binding protein 2
• NCBI Official Synonym Full Names: Y box binding protein 2
• NCBI Official Symbol: YBX2
• NCBI Official Synonym Symbols: DBPC; MSY2; CSDA3; CONTRIN
• NCBI Protein Information: Y-box-binding protein 2; MSY2 homolog; DNA-binding protein C; germ cell specific Y-box binding protein; germ cell-specific Y-box-binding protein
• UniProt Protein Name: Y-box-binding protein 2
• UniProt Gene Name: YBX2
• UniProt Synonym Gene Names: CSDA3; MSY2; Dbpc
• UniProt Entry Name: YBOX2_HUMAN

NCBI Description

This gene encodes a nucleic acid binding protein which is highly expressed in germ cells. The encoded protein binds to a Y-box element in the promoters of certain genes but also binds to mRNA transcribed from these genes. Pseudogenes for this gene are located on chromosome 10 and 15. [provided by RefSeq, Feb 2012]

Uniprot Description

YBX2: Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: polysome; cytoplasm; nucleolus; nucleus

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding; DNA binding; translation regulator activity; chromatin binding; lipid binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; translational attenuation; mRNA stabilization; negative regulation of translation; spermatogenesis; negative regulation of binding; oocyte development; spermatid development