Western Blot (WB)
(Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat MST4 Polyclonal Antibody | anti-RP6-213H19.1 antibody
Goat anti-MST4 Antibody
Western Blot: Approx 50kDa band observed in lysates of cell lines HEK293, HepG2 and Jurkat (calculated MW of 46.5kDa according to NP_057626.2). Recommended concentration: 0.5-1.5ug/ml.
Western Blot (WB)
(Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
MST4: a S/T protein kinase of the STE20 family. Mst kinases regulate processes such as apoptosis, morphogenesis, and cytoskeletal rearrangements, and are upstream activators of MAPK pathways that. The amino-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast STE20 kinase and other p21-activated kinases. otential mediator of cell growth. Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-178, possibly as a consequence of stabilization of dimer formation. May also be activated by C-terminal cleavage.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE20 family; YSK subfamily
Chromosomal Location of Human Ortholog: Xq26.2
Cellular Component: apical plasma membrane; centrosome; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; Golgi-associated vesicle; nucleus; perinuclear region of cytoplasm
Molecular Function: ATP binding; identical protein binding; magnesium ion binding; protein binding; protein homodimerization activity; protein kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: cell structure disassembly during apoptosis; microvillus biogenesis; mitotic cell cycle; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell differentiation; response to hydrogen peroxide
Research Articles on RP6-213H19.1
Similar Products
Product Notes
The RP6-213H19.1 stk26 (Catalog #AAA420596) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MST4 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MST4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 50kDa band observed in lysates of cell lines HEK293, HepG2 and Jurkat (calculated MW of 46.5kDa according to NP_057626.2). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the RP6-213H19.1 stk26 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KLIEKFQKCS ADESP. It is sometimes possible for the material contained within the vial of "MST4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
