Rabbit MSK1 Polyclonal Antibody | anti-MSK1 antibody

MSK1 (Q577) Polyclonal Antibody

Cat. Num. AAA3001729

• Gene Names: RPS6KA5; MSK1; RLPK; MSPK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MSK1; Polyclonal Antibody; MSK1 (Q577) Polyclonal Antibody; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL; RPS6KA5; anti-MSK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MSK1 (Q577) polyclonal antibody detects endogenous levels of MSK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 802

• Applicable Applications for anti-MSK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 551-600 of Human MSK1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MSK1  (Q577)  pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MSK1 (Q577) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of MSK1 (Q577) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing Nucleus and Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-MSK1 antibody

MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress. Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain.

NCBI and Uniprot Product Information

• NCBI GI #: 32528295
• NCBI GeneID: 9252
• NCBI Accession #: NP_004746.2
• NCBI GenBank Nucleotide #: NP_004746.2
• UniProt Accession #: O75582
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-5 isoform a
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A5
• NCBI Official Symbol: RPS6KA5
• NCBI Official Synonym Symbols: MSK1; RLPK; MSPK1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-5
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-5
• UniProt Gene Name: RPS6KA5
• UniProt Synonym Gene Names: MSK1; S6K-alpha-5; RSKL
• UniProt Entry Name: KS6A5_HUMAN

Uniprot Description

MSK1: an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Enzyme activity requires the presence of both kinase domains. Is phosphorylated and activated by Erk as well as p38 MAPK in response to growth factors and cellular stress.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; AGC group; RSK family; MSK subfamily

Chromosomal Location of Human Ortholog: 14q31-q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: axon guidance; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; histone phosphorylation; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; inflammatory response; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; activation of CREB transcription factor; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of cytokine production; toll-like receptor signaling pathway; innate immune response; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent