Goat anti-Human, Mouse MSH4, internal Polyclonal Antibody | anti-MSH4 antibody

MSH4, internal (RP11-262K24.2, mutS homolog 4)

Cat. Num. AAA622924

• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: MSH4; internal; Polyclonal Antibody; internal (RP11-262K24.2; mutS homolog 4); Anti -MSH4; anti-MSH4 antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human MSH4. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-MSH4 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: Peptide ELISA: 1:64,000.; Western Blot: Preliminary experiments gave bands at approx 60kD and 35kD in Human Testis, Ovary and Uterus lysates and in Mouse and Rat Testis lysates after 0.1ug/ml antibody staining. Optimal dilutions to be determined by the researcher.
• Immunogen: A synthetic peptide from the C-terminus of aa--ETNSSTFMKEMKE.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-MSH4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 36949366
• NCBI GeneID: 4438
• NCBI Accession #: NP_002431.2
• NCBI GenBank Nucleotide #: NM_002440.3
• UniProt Accession #: O15457; Q5T4U6; Q8NEB3; Q9UNP8; Q5ZEZ0
• Molecular Weight: 104,756 Da
• NCBI Official Full Name: mutS protein homolog 4
• NCBI Official Synonym Full Names: mutS homolog 4 (E. coli)
• NCBI Official Symbol: MSH4
• NCBI Protein Information: mutS protein homolog 4; hMSH4; OTTHUMP00000011349
• UniProt Protein Name: MutS protein homolog 4
• Protein Family: MutS protein
• UniProt Gene Name: MSH4
• UniProt Entry Name: MSH4_HUMAN

NCBI Description

This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair. [provided by RefSeq, Aug 2011]

Uniprot Description

MSH4: Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis. Belongs to the DNA mismatch repair MutS family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: synaptonemal complex; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; damaged DNA binding; ATP binding; mismatched DNA binding

Biological Process: homologous chromosome segregation; ovarian follicle development; meiotic recombination; female gamete generation; spermatogenesis; chiasma formation; meiotic mismatch repair