Aldose reductase Active Protein | AKR1B1 active protein

Aldo-keta reductase family 1, member B1

Cat. Num. AAA203284

• Gene Names: AKR1B1; AR; ADR; ALR2; ALDR1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Aldose reductase; Aldo-keta reductase family 1; member B1; Aldo-keto reductase family1 member B1; AKR1B1; ADR; ALDR1; ALR2; AR; Aldo-keto reductase family1; member B1 AKR1B 1; Aldehyde reductase; aldehyde reductase 1; aldo-keto reductase family 1; member B1 aldr 1; aldr-1; Lii5-2 CTCL tumor antigen; low Km aldose reductase.; AKR1B1 active protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
• Sequence Length: 316

• Applicable Applications for AKR1B1 active protein: SDS-PAGE
• Antigen Species: Human
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is >800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AKR1B1 active protein

Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AKR1B1 active protein

Redox Proteins

References

Ruiz FX., et al: (2009) Chem Biol Interact.178 (1-3); Donaqhue KC., et al: Diabet Med. 22 (10):1315-20;

NCBI and Uniprot Product Information

• NCBI GI #: 4502049
• NCBI GeneID: 231
• NCBI Accession #: NP_001619
• NCBI GenBank Nucleotide #: NM_001628.3
• UniProt Accession #: P15121; Q5U031; Q6FGA4; Q6ICP2; Q9BS21; Q9UCI9; B2R8N3
• Molecular Weight: 35.8kDa (316aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: aldose reductase isoform 1
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member B
• NCBI Official Symbol: AKR1B1
• NCBI Official Synonym Symbols: AR; ADR; ALR2; ALDR1
• NCBI Protein Information: aldose reductase
• UniProt Protein Name: Aldose reductase
• Protein Family: Aldose reductase
• UniProt Gene Name: AKR1B1
• UniProt Synonym Gene Names: ALDR1; AR

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Uniprot Description

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.