Western Blot (WB)
(Figure 1: Western blot analysis using TRIP6 mAb against human TRIP6 recombinant protein. (Expected MW is 44.4 kDa))
Mouse anti-Human TRIP6 Monoclonal Antibody | anti-TRIP6 antibody
TRIP6 Antibody
Gene Names
TRIP6; OIP1; OIP-1; ZRP-1; TRIP-6; TRIP6i2
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
TRIP6; Monoclonal Antibody; TRIP6 Antibody; MGC10556; MGC10558; MGC29959; MGC3837; MGC4423; OIP-1; OIP1; OPA interacting protein 1; OPA-interacting protein 1; Thyroid hormone receptor interactor 6; Thyroid receptor interacting protein 6; Thyroid receptor-interacting protein 6; TR-interacting protein 6; TRIP-6; Trip6; TRIP6_HUMAN; TRIP6i2; ZRP 1; ZRP-1; Zyxin-related protein 1; anti-TRIP6 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
TRIP6 antibody detects endogenous levels of total TRIP6
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
476
Applicable Applications for anti-TRIP6 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human TRIP6 expressed in E. Coli
Subcellular Location
Cytoplasm > Cytoskeleton. Cell Junction > Focal Adhesion. Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
Tissue Specificity
Abundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using TRIP6 mAb against human TRIP6 recombinant protein. (Expected MW is 44.4 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using TRIP6 mAb against human TRIP6 recombinant protein. (Expected MW is 44.4 kDa))
Related Product Information for anti-TRIP6 antibody
Description: This gene is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA)-dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Function: Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
Subunit Structure: Specifically interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone (PubMed:14688263). Interacts (via the third LIM domain and C-terminus) with PTPN13 (via the second PDZ domain) (PubMed:10400701, PubMed:17591779, PubMed:19017743, PubMed:10826496). Interacts (via the second LIM domain or via the third LIM domain plus C-terminus) with PDLIM4 (via PDZ domain) (PubMed:10826496). Found in a complex with PTPN13 and PDLIM4 (By similarity). Interacts with SVIL isoform 2 (PubMed:16880273). Interacts with LPAR2 but not other LPA receptors (PubMed:14688263). Interacts with PRKAA2 (PubMed:16624523). Interacts with MAGI1 (PubMed:19017743). Interacts with SCRIB (PubMed:16137684). In case of infection, interacts with S.typhimurium protein sseI (PubMed:17095609).
Post-translational Modifications: Phosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13.
Similarity: The LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI. Belongs to the zyxin/ajuba family.
Function: Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
Subunit Structure: Specifically interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone (PubMed:14688263). Interacts (via the third LIM domain and C-terminus) with PTPN13 (via the second PDZ domain) (PubMed:10400701, PubMed:17591779, PubMed:19017743, PubMed:10826496). Interacts (via the second LIM domain or via the third LIM domain plus C-terminus) with PDLIM4 (via PDZ domain) (PubMed:10826496). Found in a complex with PTPN13 and PDLIM4 (By similarity). Interacts with SVIL isoform 2 (PubMed:16880273). Interacts with LPAR2 but not other LPA receptors (PubMed:14688263). Interacts with PRKAA2 (PubMed:16624523). Interacts with MAGI1 (PubMed:19017743). Interacts with SCRIB (PubMed:16137684). In case of infection, interacts with S.typhimurium protein sseI (PubMed:17095609).
Post-translational Modifications: Phosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13.
Similarity: The LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI. Belongs to the zyxin/ajuba family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 50.3 kDa
Predicted: 51 kDa
Predicted: 51 kDa
NCBI Official Full Name
thyroid receptor-interacting protein 6
NCBI Official Synonym Full Names
thyroid hormone receptor interactor 6
NCBI Official Symbol
TRIP6
NCBI Official Synonym Symbols
OIP1; OIP-1; ZRP-1; TRIP-6; TRIP6i2
NCBI Protein Information
thyroid receptor-interacting protein 6
UniProt Protein Name
Thyroid receptor-interacting protein 6
Protein Family
UniProt Gene Name
TRIP6
UniProt Synonym Gene Names
OIP1; TR-interacting protein 6; TRIP-6; OIP-1; ZRP-1
NCBI Description
This gene is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA)-dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
Research Articles on TRIP6
Similar Products
Product Notes
The TRIP6 trip6 (Catalog #AAA9602303) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRIP6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIP6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the TRIP6 trip6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIP6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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