Rabbit MRPL16 Polyclonal Antibody | anti-MRPL16 antibody

Anti-MRPL16 Antibody

Cat. Num. AAA8216153

• Gene Names: MRPL16; L16mt; MRP-L16; PNAS-111
• Reactivity: Human, Monkey, Porcine
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: MRPL16; Polyclonal Antibody; Anti-MRPL16 Antibody; 39S ribosomal protein L16; mitochondrial; L16mt; MRP-L16; anti-MRPL16 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Porcine
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of MRPL16 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 251

• Applicable Applications for anti-MRPL16 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB (1/500 - 1/1000), IHC (1/100 - 1/200)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MRPL16. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MRPL16 expression in HepG2 (A), HeLa (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MRPL16 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Related Product Information for anti-MRPL16 antibody

Rabbit polyclonal antibody to MRPL16

NCBI and Uniprot Product Information

• NCBI GI #: 8923448
• NCBI GeneID: 54948
• NCBI Accession #: NP_060310.1
• NCBI GenBank Nucleotide #: NM_017840.3
• UniProt Accession #: Q9NX20; Q9BYD0; Q9HB70
• Molecular Weight: 28,449 Da
• NCBI Official Full Name: 39S ribosomal protein L16, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L16
• NCBI Official Symbol: MRPL16
• NCBI Official Synonym Symbols: L16mt; MRP-L16; PNAS-111
• NCBI Protein Information: 39S ribosomal protein L16, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L16, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL16
• UniProt Synonym Gene Names: L16mt; MRP-L16
• UniProt Entry Name: RM16_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL16: Component of the large subunit of mitochondrial ribosome. Belongs to the ribosomal protein L16P family.

Protein type: Mitochondrial; RNA-binding

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function: rRNA binding; structural constituent of ribosome

Biological Process: mitochondrial translation; organelle organization and biogenesis