Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Mothers Against Decapentaplegic Homolog 6 Polyclonal Antibody | anti-Smad6 antibody
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 6 (Smad6)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- QELKTVTYSL LKRLKERSLD TLLEAVESRG GVPGGCVLVP RADLRLGGQP APPQLLLGRL FRWPDLQHAV ELKPLCGCHS FAAAADGPTV CCNPYHFSRL CGPESPPPPY SRLSPPDQHK PLDLSDSTLS YTETEATNSL ITAPGEFSDA SMSPDATKPS HWCSVAYWEH RTRVGRLYAV YDQAVSIFYD LPQGSGFCLG QLNLEQRSES VRRTRSKIGF GILLSKEPDG VWAYNRGEHP IFVNSPTLDA PGGRALVVRK VPPGYSIKVF D
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded spleen tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded spleen tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Spleen Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Spleen Tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]
Uniprot Description
SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 15q22.31
Cellular Component: transcription factor complex; protein complex; cytoplasm; cytosol; nucleus
Molecular Function: identical protein binding; protein binding; metal ion binding; ubiquitin protein ligase binding; chromatin binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; transcription factor activity
Biological Process: fat cell differentiation; BMP signaling pathway; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; transcription, DNA-dependent; ureteric bud development; response to estrogen stimulus; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; cell-substrate adhesion; negative regulation of BMP signaling pathway; negative regulation of apoptosis
Disease: Aortic Valve Disease 2
Research Articles on Smad6
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Product Notes
The Smad6 smad6 (Catalog #AAA2028496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 6 (Smad6) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mothers Against Decapentaplegic Homolog 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the Smad6 smad6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- QELKTVTYSL LKRLKERSLD TLLEAVESRG GVPGGCVLVP RADLRLGGQP APPQLLLGRL FRWPDLQHAV ELKPLCGCHS FAAAADGPTV CCNPYHFSRL CGPESPPPPY SRLSPPDQHK PLDLSDSTLS YTETEATNSL ITAPGEFSDA SMSPDATKPS HWCSVAYWEH RTRVGRLYAV YDQAVSIFYD LPQGSGFCLG QLNLEQRSES VRRTRSKIGF GILLSKEPDG VWAYNRGEHP IFVNSPTLDA PGGRALVVRK VPPGYSIKVF D. It is sometimes possible for the material contained within the vial of "Mothers Against Decapentaplegic Homolog 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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