Mothers Against Decapentaplegic Homolog 6 Recombinant Protein | Smad6 recombinant protein

Recombinant Mothers Against Decapentaplegic Homolog 6 (Smad6)

Cat. Num. AAA2031670

• Gene Names: SMAD6; AOVD2; MADH6; MADH7; HsT17432
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Mothers Against Decapentaplegic Homolog 6; Recombinant Mothers Against Decapentaplegic Homolog 6 (Smad6); Smad6 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.1 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- PESDC ETVTCCLFSE RDAAGAPRDS GDPQARQSPE PEEGGGPRSR EARSRLLLLE QELKTVTYSL LKRLKERSLD TLLEAVESRG GVPGGCVLVP RADLRLGGQP APPQLLLGRL FRWPDLQHAV ELKPLCGCHS FTAAADGPTV CCNPYHFSRL CGPESPPPPY SRLSPPDQYK PLDLSDSTLS YTETEATNSLITAPGEFSDA SMSPDATKPS HWCSVAYWEH RTRVGRLYAV YDQAVSIFYD LPQG
• Sequence Length: 496

• Applicable Applications for Smad6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 30.8kDa
• Accurate Molecular Mass (KD): 31kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Pro116~Gly364
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 92859871
• NCBI GeneID: 4091
• NCBI Accession #: NP_005576.3
• NCBI GenBank Nucleotide #: NM_005585.4
• Molecular Weight: 35,467 Da
• NCBI Official Full Name: mothers against decapentaplegic homolog 6
• NCBI Official Synonym Full Names: SMAD family member 6
• NCBI Official Symbol: SMAD6
• NCBI Official Synonym Symbols: AOVD2; MADH6; MADH7; HsT17432
• NCBI Protein Information: mothers against decapentaplegic homolog 6
• UniProt Protein Name: Mothers against decapentaplegic homolog 6
• Protein Family: Mothers against decapentaplegic
• UniProt Gene Name: SMAD6
• UniProt Synonym Gene Names: MADH6; MAD homolog 6; Mothers against DPP homolog 6; SMAD 6; Smad6; hSMAD6
• UniProt Entry Name: SMAD6_HUMAN

NCBI Description

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]

Uniprot Description

SMAD6: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions. Interacts with NEDD4L. Interacts with WWP1. Interacts with RNF111 and AXIN1. Interacts with TGF-beta type I receptor superfamily members, SMAD1, HOXC8 and HOXC9. Interacts with STAMBP and PRKX. Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue. Belongs to the dwarfin/SMAD family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: transcription factor complex; protein complex; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; ubiquitin protein ligase binding; metal ion binding; chromatin binding; transforming growth factor beta receptor, inhibitory cytoplasmic mediator activity; transcription factor activity

Biological Process: fat cell differentiation; transcription, DNA-dependent; cell-substrate adhesion; negative regulation of BMP signaling pathway; BMP signaling pathway; negative regulation of cell proliferation; zygotic determination of dorsal/ventral axis; ureteric bud development; response to estrogen stimulus; transforming growth factor beta receptor signaling pathway; immune response; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of apoptosis

Disease: Aortic Valve Disease 2