Goat MGLL / MGL Polyclonal Antibody | anti-MGLL antibody

Goat Polyclonal to Human MGLL / MGL

Cat. Num. AAA247885

• Gene Names: MGLL; MGL; HUK5; MAGL; HU-K5
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rabbit, Rat; Predicted Reactivity: Dog, Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MGLL / MGL; Polyclonal Antibody; Goat Polyclonal to Human MGLL / MGL; Anti-MGLL / MGL Antibody (Internal) IHC-plus; MGLL; HUK5; Lysophospholipase homolog; MGL; Monoacylglycerol lipase; HU-K5; Lysophospholipase-like; MAGL; Monoglyceride lipase; Human MGLL; anti-MGLL antibody

• Host: Goat
• Reactivity: Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rabbit, Rat; Predicted Reactivity: Dog, Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human MGLL. This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 313

• Applicable Applications for anti-MGLL antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.5 - 1.5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QDLPHLVNADGQY from an internal region (near N-terminus) of human MGLL (NP_009214.1; NP_001003794.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Rabbit (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: MGLL / MGL antibody was raised against synthetic peptide C-QDLPHLVNADGQY from an internal region (near N-terminus) of human MGLL (NP_009214.1; NP_001003794.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Rabbit (100%); Dog, Pig, Opossum (92%); Horse (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MGLL antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MGLL antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody (0.5 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-MGLL antibody

MAG Lipase is a lipase that functions together with hormone-sensitive lipase to hydrolyze intracellular triglyceride stores in adipocytes and other cell types to form fatty acids and glycerol. MAG lipase also works with lipoprotein lipase to hydrolyze monoglycerides resulting from degradation of lipoprotein triglycerides. It is localized in the enteric nervous system to function in the regulation of levels of endocannabinoids, and regulates intestinal motility and has a role in digestion.

NCBI and Uniprot Product Information

• NCBI GI #: 6005786
• NCBI GeneID: 11343
• NCBI Accession #: NP_009214.1
• NCBI GenBank Nucleotide #: NM_007283.6
• UniProt Accession #: Q99685; Q6IBG9; Q96AA5; B3KRC2; B7Z9D1
• Molecular Weight: 31,149 Da
• NCBI Official Full Name: monoglyceride lipase isoform 1
• NCBI Official Synonym Full Names: monoglyceride lipase
• NCBI Official Symbol: MGLL
• NCBI Official Synonym Symbols: MGL; HUK5; MAGL; HU-K5
• NCBI Protein Information: monoglyceride lipase; monoacylglycerol lipase; lysophospholipase homolog
• UniProt Protein Name: Monoglyceride lipase
• Protein Family: Monoglyceride lipase
• UniProt Gene Name: MGLL
• UniProt Synonym Gene Names: MGL; MAGL
• UniProt Entry Name: MGLL_HUMAN

NCBI Description

This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

Protein type: Phospholipase; Lipid Metabolism - glycerolipid; EC 3.1.1.23

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol

Molecular Function: protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity

Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; triacylglycerol catabolic process; regulation of inflammatory response; arachidonic acid metabolic process; regulation of sensory perception of pain; lipid metabolic process; blood coagulation; inflammatory response; fatty acid biosynthetic process; acylglycerol catabolic process