Rabbit anti-Human Monocarboxylate Transporter 1 Polyclonal Antibody | anti-SLC16A1 antibody

Monocarboxylate Transporter 1 (MCT1, MOT1)

Cat. Num. AAA616208

• Gene Names: SLC16A1; MCT; HHF7; MCT1; FLJ36745; MGC44475
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Monocarboxylate Transporter 1; Polyclonal Antibody; Monocarboxylate Transporter 1 (MCT1; MOT1); Anti -Monocarboxylate Transporter 1 (MCT1; anti-SLC16A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Monocarboxylate Transporter 1 (MCT1).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.5, 0.1% BSA. Reconstitute with 100ul sterile PBS

• Applicable Applications for anti-SLC16A1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Dilution: Western Blot: 1-5ug/ml using ECL.; Immunohistochemistry: 3-10ug/ml. neutral buffered formaldehyde fixed tissue.; ELISA: 1:10,000-1:100,000 using 50-100ng control peptide /well
• Immunogen: A synthetic peptide corresponding to 19aa near the C-terminus, cytoplasmic domain of human MCT1, conjugated to KLH. Species Sequence Homology: hamster; 63%, mouse; 57%
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC16A1 antibody

Monocarboxylate such as lactate and pyruvate play an important role in cellular metabolism. Lactic acid is produced as the end product of glycolysis. Some tissues, such as white skeletal muscle and, red blood cells, use this pathway to generate most of their ATP under normal physiological conditions. All tissues become dependent on this pathway during abnormal conditions such as hypoxia and ischaemia. Lactic acid, produced during normal glycolysis, must be transported out of cells to sustain maintain high rate of glycolysis. Failure to export lactic acid leads to accumulation of cellular lactic acid followed by an increase in pH and inhibition of glycolysis. Some tissues, such as brain, heart, and red skeletal muscle, readily oxidize lactic acid, and must import lactic acid into the cells. Lactic acid transport is mediated by a group of proton-linked membrane transporters called monocarboxylic acid transporters (MCTs). At least 9 MCT-related proteins (MCT1-9) have been identified in mammals that are expressed in a tissue specific manner. MCT1 (also known as MOT1 or SLC16A1 or MEV; mouse 493aa, rat 494aa, human 500aa; ~95% identity) is a membrane protein containing 12 transmembrane proteins. MCT1 is most closely related to MCT2 (~65% identity, whereas homolog with other MCT2-MCT8 isoforms is less (~35-53%). MCT1 has very wide tissue distribution. MCT1/MOT1 is ubiquitously expressed but is especially prominent in heart and red muscle. It is upregulated in response to increased work, suggesting an important role in lactic acid oxidation. It is the major isoform in tumor cell and erythrocytes.

Product Categories/Family for anti-SLC16A1 antibody

Antibodies; Abs to Monocarboxylate Transporter

NCBI and Uniprot Product Information

• NCBI GI #: 115583685
• NCBI GeneID: 6566
• NCBI Accession #: NP_003042.3
• NCBI GenBank Nucleotide #: NM_003051.3
• UniProt Accession #: P53985; Q5T8R6; Q9NSJ9; Q49A45; Q5T8R3; Q5T8R4; Q5T8R5
• Molecular Weight: 53,944 Da
• NCBI Official Full Name: monocarboxylate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 16, member 1 (monocarboxylic acid transporter 1)
• NCBI Official Symbol: SLC16A1
• NCBI Official Synonym Symbols: MCT; HHF7; MCT1; FLJ36745; MGC44475
• NCBI Protein Information: monocarboxylate transporter 1; MCT 1; OTTHUMP00000013960; OTTHUMP00000013961; solute carrier family 16 member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1
• UniProt Protein Name: Monocarboxylate transporter 1
• Protein Family: Monocarboxylate transporter
• UniProt Gene Name: SLC16A1
• UniProt Synonym Gene Names: MCT1
• UniProt Entry Name: MOT1_HUMAN

NCBI Description

The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.

Uniprot Description

MCT1: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Defects in SLC16A1 are the cause of symptomatic deficiency in lactate transport (SDLT); also known as erythrocyte lactate transporter defect. Deficiency of lactate transporter may result in an acidic intracellular environment created by muscle activity with consequent degeneration of muscle and release of myoglobin and creatine kinase. This defect might compromise extreme performance in otherwise healthy individuals. Defects in SLC16A1 are the cause of familial hyperinsulinemic hypoglycemia type 7 (HHF7); also known as exercise-induced hyperinsulinemic hypoglycemia. HHF7 is a dominantly inherited hypoglycemic disorder characterized by inappropriate insulin secretion during anaerobic exercise or on pyruvate load. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Protein type: Transporter, SLC family; Mitochondrial; Membrane protein, multi-pass; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p12

Cellular Component: centrosome; mitochondrion; membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: mevalonate transmembrane transporter activity; protein homodimerization activity; monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity

Biological Process: response to food; monocarboxylic acid transport; glucose homeostasis; cellular metabolic process; centrosome organization and biogenesis; mevalonate transport; lipid metabolic process; pyruvate metabolic process; blood coagulation; leukocyte migration; transmembrane transport; regulation of insulin secretion; behavioral response to nutrient

Disease: Erythrocyte Lactate Transporter Defect; Monocarboxylate Transporter 1 Deficiency; Hyperinsulinemic Hypoglycemia, Familial, 7