Rabbit anti-Human MOCS1 Polyclonal Antibody | anti-MOCS1 antibody

MOCS1, NT (MOCS1, Molybdenum cofactor biosynthesis protein 1, Cell migration-inducing gene 11 protein, Molybdenum cofactor synthesis-step 1 protein A-B, Molybdenum cofactor biosynthesis protein A, Molybdenum cofactor biosynthesis protein C) (PE)

Cat. Num. AAA6321613

• Gene Names: MOCS1; MIG11; MOCOD
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MOCS1; Polyclonal Antibody; NT (MOCS1; Molybdenum cofactor biosynthesis protein 1; Cell migration-inducing gene 11 protein; Molybdenum cofactor synthesis-step 1 protein A-B; Molybdenum cofactor biosynthesis protein A; Molybdenum cofactor biosynthesis protein C) (PE); Molybdenum cofactor biosynthesis protein C; anti-MOCS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MOCS1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human MOCS1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MOCS1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MOCS1 antibody detected the MOCS1 protein (arrow).)

Related Product Information for anti-MOCS1 antibody

Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However, only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA, whereas additional splice variants, whose full-length natures have yet to be determined, are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency, type A. A related pseudogene has been identified on chromosome 16.

Product Categories/Family for anti-MOCS1 antibody

Antibodies; Proteins

References

Sass, J.O., et al. Brain Dev. (2009). In press: Arenas, M., et al. J. Inherit. Metab. Dis. 32(4):560-569(2009). Ichida, K., et al. Nucleosides Nucleotides Nucleic Acids 25 (9-11), 1087-1091 (2006): Macaya, A., et al. Neuropediatrics 36(6):389-394(2005). Leimkuhler, S., et al. Hum. Genet. 117(6):565-570(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 116734668
• NCBI GeneID: 4337
• NCBI Accession #: NP_001068566.1
• NCBI GenBank Nucleotide #: NM_001075098.3
• UniProt Accession #: Q9NZB8; O14940; O14941; O75710; Q5J7W0; Q5TCE1; Q5TCE2; Q5TCE6; Q5TCE9; Q5TCF0; B3KPT7; B4DTP1
• Molecular Weight: 70,105 Da
• NCBI Official Full Name: molybdenum cofactor biosynthesis protein 1 isoform 4
• NCBI Official Synonym Full Names: molybdenum cofactor synthesis 1
• NCBI Official Symbol: MOCS1
• NCBI Official Synonym Symbols: MIG11; MOCOD
• NCBI Protein Information: molybdenum cofactor biosynthesis protein 1; MOCS1A enzyme; migration-inducing gene 11 protein; cell migration-inducing gene 11 protein; molybdenum cofactor biosynthesis protein A; molybdenum cofactor synthesis-step 1 protein A-B
• UniProt Protein Name: Molybdenum cofactor biosynthesis protein 1
• UniProt Gene Name: MOCS1
• UniProt Entry Name: MOCS1_HUMAN

NCBI Description

Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However, only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA, whereas additional splice variants, whose full-length natures have yet to be determined, are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency, type A. A related pseudogene has been identified on chromosome 16. [provided by RefSeq, Jan 2010]

Uniprot Description

Function: Isoform MOCS1A and isoform MOCS1B probably form a complex that catalyzes the conversion of 5'-GTP to cyclic pyranopterin monophosphate (cPMP or molybdopterin precursor Z). Ref.8

Catalytic activity: GTP = cyclic pyranopterin phosphate + diphosphate. Ref.8

Cofactor: Binds 2 4Fe-4S clusters. Binds 1 4Fe-4S cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 4Fe-4S cluster coordinated with 3 cysteines and the GTP-derived substrate. Ref.10

Pathway: Cofactor biosynthesis; molybdopterin biosynthesis.

Subunit structure: Isoform MOCS1A and isoform MOCS1B probably form a heterooligomer

Probable.

Tissue specificity: Isoform MOCS1A and isoform 2 are widely expressed. Ref.1 Ref.9

Post-translational modification: Isoform MOCS1A, isoform 2 and isoform 3 are probably thiocarboxylated at their C-terminus. Thiocarboxylation probably plays a central role in molybdenum cofactor biosynthesis, since mutagenesis of the last 2 Gly residues of isoform MOCS1A abolishes the catalytic activity of the enzyme. Thiocarboxylation is absent in isoform MOCS1B, which lacks the C-terminal Gly residue.

Involvement in disease: Molybdenum cofactor deficiency type A (MOCOD type A) [MIM:252150]: Autosomal recessive disease which leads to the pleiotropic loss of all molybdoenzyme activities and is characterized by severe neurological damage, neonatal seizures and early childhood death.Note: The disease is caused by mutations affecting the gene represented in this entry.

Miscellaneous: The MOCS1 locus has initially been reported to produce MOCS1A and MOCS1B from non-overlapping reading frames within a bicistronic transcript. However, only isoform MOCS1A seems to be translated from the bicistronic transcript. Isoform MOCS1B seems to be translated from a monocistronic mRNA that is derived by alternative splicing.

Sequence similarities: In the C-terminal section; belongs to the MoaC family.In the N-terminal section; belongs to the MoaA/NifB/PqqE family.

Sequence caution: The sequence AAB87524.1 differs from that shown. Reason: Alternative splicing in the MOCS1A-MOCS1B joining region.The sequence AAS00489.1 differs from that shown. Reason: Erroneous initiation. The sequence CAC44526.1 differs from that shown. Reason: Alternative splicing in the MOCS1A-MOCS1B joining region.The sequence CAI20007.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI20012.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI20013.1 differs from that shown. Reason: Erroneous gene model prediction.