Rabbit anti-Human MOB4 Polyclonal Antibody | anti-MOB4 antibody

Anti-MOB4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106386

• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: MOB4; Polyclonal Antibody; Anti-MOB4 Antibody; Rabbit Polyclonal; MOB4 Antibody; Rabbit PAb; Antigen Affinity Purified; MOB family member 4; phocein; Anti-2C4D Antibody; Anti-CGI-95 Antibody; Anti-MOB1 Antibody; Anti-MOB3 Antibody; Anti-MOBKL3 Antibody; Anti-PHOCN Antibody; Anti-PREI3 Antibody; anti-MOB4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MOB4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MOB4 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human MOB4 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human MOB4/PREI3/MOBKL3 isoform 1 (Q9Y3A3-1; Val 2-Ala 225). MOB4 specific IgG was purified by Human MOB4 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MOBKL3 rabbit polyclonal antibody at 1:500 dilutionLane A: HT-29 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa(We are unsure as to the identity of these extra bands.))

Immunoprecipitation (IP)

(MOBKL3 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate1 uL anti-MOBKL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MOBKL3 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)

Related Product Information for anti-MOB4 antibody

MOB4 (MOB Family Member 4, Phocein) is a Protein Coding gene. The MOB4 gene, located on 2q33.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. MOB4 belongs to the MOB1/phocein family. 3 alternatively spliced human isoforms have been reported. This gene was identified based on its similarity with the mouse counterpart. Studies of the mouse counterpart suggest that the expression of this gene may be regulated during oocyte maturation and preimplantation following zygotic gene activation. MOB4 may also play a role in membrane trafficking, specifically in membrane budding reactions. MOB4 is widely expressed in the adrenal, esophagus, and other tissues. Diseases associated with MOB4 include Cerebral Cavernous Malformations 3 and Febrile Seizures, Familial, 6.

References

Hirabayashi S, et al. (2008) Threonine 74 of mob1 is a putative key phosphorylation site by mst2 to form the scaffold to activate nuclear dbf2-related kinase 1. Oncogene 27 (31): 4281-4292.Chen M, et al. (2018) The mst4-mob4 complex disrupts the mst1-mob1 complex in the hippo-yap pathway and plays a pro-oncogenic role in pancreatic cancer. J Biol Chem 293 (37): 14455-14469.Bailly YJ, et al. (2007) Phocein: A potential actor in vesicular trafficking at purkinje cell dendritic spines. Cerebellum 6 (4): 344-352.