Human Renin ELISA Kit | REN elisa kit

Human Renin ELISA Kit

Cat. Num. AAA9426249

• Gene Names: REN; HNFJ2
• Reactivity: Human
• Synonyms: Renin; Human Renin ELISA Kit; Angiotensinogenase; REN; 3.4.23.15; REN elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Renin. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 3.12 pg/mL -2000 pg/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for REN elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human renin concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Renin. Standards or samples are then added to the appropriate microtiter plate wells with a biotinconjugated antibody preparation specific for Renin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Renin, biotinconjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Renin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506475
• NCBI GeneID: 5972
• NCBI Accession #: NP_000528.1
• NCBI GenBank Nucleotide #: NM_000537.3
• UniProt Accession #: P00797; Q6FI38; Q6T5C2
• Molecular Weight: 44,726 Da
• NCBI Official Full Name: renin preproprotein
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin
• UniProt Protein Name: Renin
• Protein Family: Renin
• UniProt Gene Name: REN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.15; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: apical part of cell; extracellular region; extracellular space; plasma membrane

Molecular Function: aspartic-type endopeptidase activity; peptidase activity; receptor binding

Biological Process: angiotensin maturation; kidney development; protein catabolic process; proteolysis; regulation of blood pressure; regulation of MAPKKK cascade

Disease: Hyperuricemic Nephropathy, Familial Juvenile, 2; Renal Tubular Dysgenesis