Rabbit MNK2 Polyclonal Antibody | anti-MNK2 antibody

MNK2 (Phospho-T249) polyclonal antibody

Cat. Num. AAA3015066

• Gene Names: MKNK2; MNK2; GPRK7
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: MNK2; Polyclonal Antibody; MNK2 (Phospho-T249) polyclonal antibody; MAP kinase-interacting serine/threonine-protein kinase 2; MAP kinase signal-integrating kinase 2; MAPK signal-integrating kinase 2; Mnk2; MKNK2; GPRK7; anti-MNK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MNK2 (Phospho-T249) polyclonal antibody detects endogenous levels of MNK2 protein only when phosphorylated at Thr249.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MNK2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic phosphopeptide derived from human MNK2 around the phosphorylation site of Threonine 249
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of MNK2 (Phospho-T249) polyclonal antibody at 1:500 dilutionLaneA: SGC7901 whole cell lysateLaneB: PC3 whole cell lysateLaneC: Jurkat whole cell lysateLaneD: The Eye tissue lysate of MouseLaneE: The Brain tissue lysate of Rat)

Related Product Information for anti-MNK2 antibody

The MAPKAP kinases (for MAP kinase activated protein kinases) are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. These include the serine/threonine kinases Rsk-1 (also designated MAPKAP kinase-1), Rsk-2 and Rsk-3, which are phosphorylated by ERK1 and ERK2. Similarly, p38 phosphorylates and activates the serine/threonine kinases MAPKAP kinase-2 and MAPKAP kinase-3 (also designated 3pK). The serine/ threonine kinases Mnk1 and Mnk2 are substrates for both ERK and p38 MAP kinases. Mnk2 exists as multiple isoforms, including Mnk2a and Mnk2b, due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 40018631
• NCBI GeneID: 2872
• NCBI Accession #: NP_060042.2
• NCBI GenBank Nucleotide #: NM_017572.3
• UniProt Accession #: Q9HBH9; Q6GPI3; Q9HBH8; Q9UHR0; Q9Y2N6
• Molecular Weight: 51,875 Da
• NCBI Official Full Name: MAP kinase-interacting serine/threonine-protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: MAP kinase interacting serine/threonine kinase 2
• NCBI Official Symbol: MKNK2
• NCBI Official Synonym Symbols: MNK2; GPRK7
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 2; MAPK signal-integrating kinase 2; G protein-coupled receptor kinase 7; MAP kinase signal-integrating kinase 2
• UniProt Protein Name: MAP kinase-interacting serine/threonine-protein kinase 2
• UniProt Gene Name: MKNK2
• UniProt Synonym Gene Names: GPRK7; MNK2; MAPK signal-integrating kinase 2; Mnk2
• UniProt Entry Name: MKNK2_HUMAN

NCBI Description

This gene encodes a member of the calcium/calmodulin-dependent protein kinases (CAMK) Ser/Thr protein kinase family, which belongs to the protein kinase superfamily. This protein contains conserved DLG (asp-leu-gly) and ENIL (glu-asn-ile-leu) motifs, and an N-terminal polybasic region which binds importin A and the translation factor scaffold protein eukaryotic initiation factor 4G (eIF4G). This protein is one of the downstream kinases activated by mitogen-activated protein (MAP) kinases. It phosphorylates the eukaryotic initiation factor 4E (eIF4E), thus playing important roles in the initiation of mRNA translation, oncogenic transformation and malignant cell proliferation. In addition to eIF4E, this protein also interacts with von Hippel-Lindau tumor suppressor (VHL), ring-box 1 (Rbx1) and Cullin2 (Cul2), which are all components of the CBC(VHL) ubiquitin ligase E3 complex. Multiple alternatively spliced transcript variants have been found, but the full-length nature and biological activity of only two variants are determined. These two variants encode distinct isoforms which differ in activity and regulation, and in subcellular localization. [provided by RefSeq, Aug 2011]

Uniprot Description

Mnk2: a ubiquituous CAM kinase of the MAPKKAPK family. Regulates translation by phosphorylating S209 of eIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. The phosphorylation of eIF4E S209 by the MNK1/2 kinases is required for eIF4E's oncogenic action. Phosphorylates Spry2 on S112 and S121, leading to the ubiquitination and degradation of Spry2. Five splice-variant isoforms have been described.

Protein type: Translation; EC 2.7.11.1; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; MAPKAPK family; MNK subfamily

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; PML body; cytoplasm; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding

Biological Process: regulation of translation; cell surface receptor linked signal transduction; hemopoiesis; protein amino acid phosphorylation