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Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using HDAC4 antibody)

Rabbit HDAC4 Polyclonal Antibody | anti-HDAC4 antibody

HDAC4 antibody

Applications
Immunofluorescence, Western Blot
Purity
Purified by immunoaffinity chromatography.
Synonyms
HDAC4; Polyclonal Antibody; HDAC4 antibody; Rabbit polyclonal HDAC4 antibody; Polyclonal HDAC4 antibody; Anti-HDAC4 antibody; HDAC-4 antibody; HDAC-4; Histone deacetylase 4 antibody; Ab 632 antibody; HDAC 4; HDAC 4 antibody; anti-HDAC4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
Purified by immunoaffinity chromatography.
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
1056
Applicable Applications for anti-HDAC4 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100-1:200
WB: 1:500-1:1000
Immunogen
HDAC4 antibody was raised in Rabbit using the peptide sequence around aa 630~634 (A-Q-S-S-P) derived from Human HDAC4 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using HDAC4 antibody)

Immunofluorescence (IF) (Immunofluorescence staining of methanol-fixed Hela cells using HDAC4 antibody)

Western Blot (WB)

(Western Blot analysis of extracts from HepG2 cells using HDAC4 antibody)

Western Blot (WB) (Western Blot analysis of extracts from HepG2 cells using HDAC4 antibody)
Related Product Information for anti-HDAC4 antibody
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Product Categories/Family for anti-HDAC4 antibody

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
116,046 Da
NCBI Official Full Name
hdac4
UniProt Protein Name
Histone deacetylase
UniProt Gene Name
hdac4

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Similar Products

Product Notes

The HDAC4 hdac4 (Catalog #AAA5309725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HDAC4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100-1:200 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HDAC4 hdac4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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