Immunofluorescence (IF)
(MBS9600153 staining SKOV3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit MMP23 Polyclonal Antibody | anti-MMP23 antibody
MMP23 Antibody
Gene Names
MMP23B; MIFR; MMP22; MIFR-1; MMP23A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MMP23; Polyclonal Antibody; MMP23 Antibody; Femalysin; Matrix metallopeptidase 21; Matrix metallopeptidase 23A (pseudogene); Matrix metallopeptidase 23A; Matrix metallopeptidase 23B; Matrix metalloproteinase 22; Matrix metalloproteinase 23; Matrix metalloproteinase 23B; Matrix metalloproteinase in the female reproductive tract; Matrix metalloproteinase-21; Matrix metalloproteinase-22; Matrix metalloproteinase-23; MIFR; MIFR-1; MIFR1; MMP 23; MMP-21; MMP-22; MMP-23; MMP21; MMP22; MMP23_HUMAN; MMP23A; MMP23B; soluble form; anti-MMP23 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MMP23 antibody detects endogenous levels of total MMP23
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
390
Applicable Applications for anti-MMP23 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human MMP23
Subcellular Location
Endoplasmic Reticulum Membrane. Membrane. A secreted form produced by proteolytic cleavage may also exist.
Tissue Specificity
Predominantly expressed in ovary, testis and prostate.
Predicted Cross Reactivity
Bovine
Similarity
Bovine (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9600153 staining SKOV3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9600153 staining SKOV3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis on SK-OV3 cell lysate using MMP23 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on SK-OV3 cell lysate using MMP23 Antibody. The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MMP23 Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Hepg2; )
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MMP23 Antibody. Lane 1: Hela treated with blocking peptide. Lane 2: Hela; Lane 3: Hepg2; )
Related Product Information for anti-MMP23 antibody
Description: MMP23B Protease. Belongs to the peptidase M10A family. 3 isoforms of the human protein are produced by alternative splicing.
Function: Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum (By similarity).
Post-translational Modifications: N-glycosylated. Proteolytic cleavage might yield an active form.
Similarity: The ShKT domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4 (By similarity). Belongs to the peptidase M10A family.
Function: Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum (By similarity).
Post-translational Modifications: N-glycosylated. Proteolytic cleavage might yield an active form.
Similarity: The ShKT domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4 (By similarity). Belongs to the peptidase M10A family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 44 kDa
Predicted: 44 kDa
Predicted: 44 kDa
NCBI Official Full Name
matrix metalloproteinase-23
NCBI Official Synonym Full Names
matrix metallopeptidase 23B
NCBI Official Symbol
MMP23B
NCBI Official Synonym Symbols
MIFR; MMP22; MIFR-1; MMP23A
NCBI Protein Information
matrix metalloproteinase-23
UniProt Protein Name
Matrix metalloproteinase-23
Protein Family
UniProt Gene Name
MMP23A
UniProt Synonym Gene Names
MMP21; MMP-23; MMP-21; MMP-22
NCBI Description
This gene (MMP23B) encodes a member of the matrix metalloproteinase (MMP) family, and it is part of a duplicated region of chromosome 1p36.3. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. This gene belongs to the more telomeric copy of the duplicated region. [provided by RefSeq, Jul 2008]
Uniprot Description
Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum ().
Research Articles on MMP23
Similar Products
Product Notes
The MMP23 mmp23a (Catalog #AAA9600153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP23 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP23 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MMP23 mmp23a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP23, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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