Rabbit MMP2 Polyclonal Antibody | anti-MMP2 antibody

MMP2 Antibody

Cat. Num. AAA416085

• Gene Names: MMP2; CLG4; MONA; CLG4A; TBE-1; MMP-II
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: MMP2; Polyclonal Antibody; MMP2 Antibody; anti-MMP2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 61

• Applicable Applications for anti-MMP2 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: On western blots look for a major band at 66 and 72kDa, corresponding to the proenzyme (72kDa) and the activated form (66kDa).; 1:250-500 for immunofluorescence staining and 1:5000 for western blotting.
• Immunogen: Matrix metalloproteinase-2 (MMP2) (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), a member of the matrix metalloproteinase (MMP) enzyme family, is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP2 degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome, Torg syndrome and nodulosis-arthropathy-osteolysis syndrome. MMP2 is secreted as an inactive proenzyme/zymogen which is activated when cleaved by extracellular proteinases. The proform has a molecular weight of 72kDa and the active form 62kDa. A C-terminus peptide (aa. 475-490) was selected which is highly conserved and unique to MMP2, and is present in both pro- and active forms. The peptide was conjugated to KLH and use to immunize rabbits. The hyperimmune serum was affinity purified on a peptide-coupled sepharose column. The purified immunoglobulin recognizes 72 kDa and 66 kDa bands corresponding to MMP2 on Western immunoblots. Some tissue extracts also show MMP2 complexes and degradation products. Polyclonal MMP2/475 antibody immunostains MMP2 specifically in various tissue preparations including paraffin sections. This antibody has been marketed for many years by other companies and is now produced by EnCor Biotechnology Inc. The HGNC name for this protein is MMP2.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-MMP2 antibody

MMP2 is a Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD (Nagase et al., 1992). Type IV collagenase is a metalloproteinase that specifically cleaves type IV collagen, the major structural component of basement membranes (120090, 120130). The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.

References

1. Collier, I. E., Wilhelm, S. M., Eisen, A. Z., Marmer, B. L., Grant, G. A., Seltzer, J. L., Kronberger, A., He, C. S., Bauer, E. A., and Goldberg, G. I. H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen. J. Biol. Chem. 263:6579-6587 (1988).2. C. Krekoski, D. Neubauer, J.B. Graham D. Muir. Metalloproteinase-dependent predegeneration in vitro enhances axonal regeneration within acellular peripheral nerve grafts. J. Neuroscience 22:10408-10415 (2002).3. D. Muir. Differences in proliferation and invasion by normal, transformed and NF1 Schwann cell cultures are influenced by matrix metalloproteinase expression. Clinical and Experimental Metastasis 13:303-314 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 88043756
• NCBI GeneID: 4313
• UniProt Accession #: P08253; Q9UCJ8; B2R6U1; B4DWH3; E9PE45
• Molecular Weight: 73,882 Da
• NCBI Official Full Name: MMP2
• NCBI Official Synonym Full Names: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• NCBI Official Symbol: MMP2
• NCBI Official Synonym Symbols: CLG4; MONA; CLG4A; TBE-1; MMP-II
• NCBI Protein Information: 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-2; matrix metalloproteinase-II
• UniProt Protein Name: 72 kDa type IV collagenase
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP2
• UniProt Synonym Gene Names: CLG4A; MMP-2
• UniProt Entry Name: MMP2_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.22 Ref.24PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.22 Ref.24Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. Ref.13 Ref.14 Ref.15 Ref.16 Ref.18 Ref.22 Ref.24

Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.

Cofactor: Binds 4 calcium ions per subunit.Binds 2 zinc ions per subunit.

Enzyme regulation: Inhibited by histatin-3 1/24 (histatin-5). Ref.17

Subunit structure: Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Ref.10 Ref.16 Ref.18 Ref.22

Subcellular location: Isoform 1: Secreted › extracellular space › extracellular matrix. Membrane. Nucleus. Note: Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes. Ref.11 Ref.20 Ref.24Isoform 2: Cytoplasm. Mitochondrion Ref.11 Ref.20 Ref.24.

Tissue specificity: Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Ref.16

Induction: Aspirin appears to inhibit expression. Ref.17 Ref.23

Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification: Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. Ref.21The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.

Involvement in disease: Torg-Winchester syndrome (TWS) [MIM:259600]: An autosomal recessive syndrome characterized by severe multicentric osteolysis with predominant involvement of the hands and feet. Additional features include coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.34 Ref.35 Ref.36

Sequence similarities: Belongs to the peptidase M10A family.Contains 3 fibronectin type-II domains.Contains 4 hemopexin repeats.